1. Lecture 07 Transcription Based on Chapter 5 Gene Expression: Transcription 1/29/2016 Copyright © 2010 Pearson Education Inc.

2. 1. Gene Expression—The Central Dogma: An Overview

3. 2a. The Transcription Process

4. 2b. The Transcription Process

5. 3a. Transcription in Bacteria - Initiation of Transcription at Promoters

6. 3b. Transcription in Bacteria - Initiation of Transcription at Promoters

7. 4. Elongation of an RNA Chain

8. 5. Termination of an RNA Chain

9. 6. Transcription in Eukaryotes

10. 7. Eukaryotic RNA Polymerases RNA polymerase I, located in the nucleolus, transcribes the three major rRNAs (28S, 18S, and 5.8S). RNA polymerase II, located in the nucleoplasm, transcribes mRNAs and some snRNAs. RNA polymerase III, located in the nucleoplasm, transcribes tRNAs, 5S rRNA, and the remaining snRNAs.

11. 8. Transcription of Protein-Coding Genes by RNA Polymerase II

12. 9. Promoters and Enhancers

13. 10. Transcription Initiation

14. 11a. The Structure and Production of Eukaryotic mRNAs

15. 11b. The Structure and Production of Eukaryotic mRNAs

16. 12. Production of Mature mRNA in Eukaryotes

17. 13. 5’ Modification

18. 14. 3’ Modification

19. 15. Introns

20. 16. Processing of Pre-mRNA to Mature mRNA

21. 17. Coupling of Pre-mRNA Processing to Transcription and to mRNA Export from the Nucleus