1. Do Now WRITE the QUESTIONS and ANSWER!!! 1. GALLERY WALK

2. GALLERY WALK PROTEIN SYTHESIS ANALOGY: A NECKLACE FACTORY

3. The RIBOSOME is like… A necklace factory. Because… The whole purpose of the factory is to make necklaces (proteins)

4. The DNA is like… The Boss Because… it tells the factory workers which type of necklaces to make.

5. The mRNA is like… The Secretary Because… it delivers the necklace recipes and orders to the factory  The Boss (DNA) tells the secretary (mRNA) which necklaces (proteins) need to be made  The secretary (mRNA) delivers the Boss’s (DNA) necklaces order to the factory (Ribosome)

6. The tRNA is like… The Delivery trucks Because… it drops off the ingredients to the factory for the correct necklaces to be made  The trucks (tRNA) drop off the ingredients – the beads (amino acids) - to make the necklaces (proteins) that the Boss (DNA) ordered

7. So… The Boss (DNA) gives the necklace orders and recipes to the Secretary (mRNA) to bring to the necklace factory (ribosomes). The Delivery Trucks (tRNA) bring the ingredients (amino acids) to the necklace factory (ribosome), where the necklaces (proteins) are made and then shipped out

8. With your partner.. Take 1 minutes to discuss the analogy and how the necklace factory relates to protein synthesis

9. But… What does this actually look like? http://www.youtube.com/watch?v=NJxobgkPEAo http://www.youtube.com/watch?v=B6O6uRb1D38

10. Annotate your drawing along with me…

11. Check Point 1) The diagram above depicts the process of ______________________________ synthesis, or how the cell makes proteins from DNA. 2) What is the name of the process happening inside the nucleus? 3) In one sentence, explain what happens in the nucleus. 4) To what organelle does mRNA go to after the first step of protein synthesis? 5) What step of protein synthesis happens here? 6) Explain what happens during the process you identified above.

12. PS Overview Practice Using your knowledge of Protein Synthesis and the Necklace Analogy, Independently complete Protein Synthesis Review practice

13. Now… We know that the mRNA brings the recipe to make the proteins And, the tRNA brings the right ingredients (amino acids) to make the proteins And, the Ribosome actually puts the ingredients together to make the proteins BUT… How does the tRNA know which amino acids to bring…?

14. How does tRNA know which amino acid to bring? 1. Breaks mRNA into groups of 3 called CODON. 2. Use a Codon Chart to TRANSLATE mRNA CODON into AMINO ACID. 3. Amino acids linked together by the RIBOSOME to form a polypeptide (PROTEIN) chain.

15. LET’S TRY IT… DNA  mRNA = TRANSCRIPTION mRNA  amino acids = TRANSLATION So… DNA  RNA  Protein

16. GUIDED PRACTICE Translation

17. TRANSCRIPTION RACE DNA Complementary Strand mRNA Amino Acids

18. TRANSCRIPTION RACE DNA CTA Complementary Strand mRNA Amino Acids

19. TRANSCRIPTION RACE DNA GCA Complementary Strand mRNA Amino Acids

20. TRANSCRIPTION RACE DNA GGT Complementary Strand mRNA Amino Acids

21. TRANSCRIPTION RACE DNA TTC CAC Complementary Strand mRNA Amino Acids

22. TRANSCRIPTION RACE DNA GTA CAT Complementary Strand mRNA Amino Acids

23. TRANSCRIPTION RACE DNA CCT GTA TCA Complementary Strand mRNA Amino Acids

24. PRACTICE Protein synthesis TAT

25. Protein Synthesis in Action Pre-lab ROLES:  DNA: Reads the codes 1-10 to the mRNA. Can’t let mRNA see the code!  mRNA: Needs a pencil and 10 long strips of paper, numbered 1-10. DNA is transcribed…A=U, T=A, G=C, and C=G. Keep codes in groups of three letters.  Ribosome: Needs a pencil and the codon word chart. Using the mRNA codons, find the corresponding words, and write a sentence. Each sentence should begin with START and finish with STOP. You will switch roles every 3 sentences (in this order…)  DNA  mRNA  mRNA  Ribosome  Ribosome  DNA

26. What does this look like in action..?

27. Autoville Practice Using your knowledge of Protein Synthesis and the Cookie Analogy, Independently complete Autoville practice