1. Bacterial Plasmids Loops of DNA found in some bacteria
Contains “special” genes that are not related to basic life functions
i.e. antibiotic resistance
Can be transferred from one bacterial cell to another
Bacterial DNA
Plasmid DNA

2. amp resistance ara B ara A ara D Plasmid from E. coli ara C O P
Gene that codes for the inhibitor protein
Operator for the arabinose operon.
Gene that gives bacteria resistance to the antibiotic ampicillin.
Promoter for the arabinose operon.
Structural genes for arabinose metabolism

3. The Arabinose Operon in normal E. coli (for arabinose metabolism)
Repressor protein
(from ara C gene) O P
ara B
ara A
ara D
Structural genes
(for arabinose metabolism)
Regulatory genes
(operator & promoter)

4. The Arabinose Operon in normal E. coli
RNA
polymerase O P
ara B
ara A
ara D

5. The Arabinose Operon in normal E. coli
ara A
ara D

6. The Arabinose Operon in normal E. coli
ara A
ara D

7. The Arabinose Operon in normal E. coli
ara A
ara D

8. The Arabinose Operon in normal E. coli
RNA
polymerase
Arabinose O P
ara B
ara A
ara D

9. The Arabinose Operon in normal E. coli
RNA
polymerase
Arabinose O P
mRNA
ara B
ara A
ara D

10. The Arabinose Operon in normal E. coli
RNA
polymerase
Arabinose O P
ara B
ara A
ara D
mRNA