2. Chapter 28

4. Protista!

5. Make a wet mount slide of the “pond” water (from your very own GCM ditch) using about 2 drops of “mucky” water Observe the protists in the water! Draw at least 6 diagrams total of protists you observe under 10X and 40X magnifications (3 in each?) Make your drawings as detailed as possible and label parts you think you can identify. Be sure to include the magnification under which you are observing. Research protists and try to classify the organisms you drew! Survey of Protists!

6. Why is “Protista” no longer considered a kingdom?

7. Most are unicellular but can be colonial or multicellular Protista

8. 1.Photosynthetic, plant-like protists (algae) 2.Ingestive, animal-like protists (protozoa) 3.Absorptive, fungus-like protists Protista

9. Some are motile, others are not; most have cilia or flagella at some point in their lives Protista

10. Life Cycles 1.Mitosis 2.Sexual reproduction 3.Meiosis and syngamy (formation of a zygote from 2 gametes) Protista

11. Habitats – most are aquatic, including oceans, ponds, lakes, damp soil, leaf litter, etc 1.Important constituents of plankton; phytoplankton (photosynthetic protists) are the bases of most marine and freshwater food webs 2.Symbionts inhabiting body fluids, tissues, and cells of hosts (relationships vary from mutualistic to parasitic) 3.Parasitic parasites include the malaria parasites (several species of plasmodium), Giardia lamblia (synonymous with Giardia intestinalis) which causes diarrhea, Trichomonas vaginalis (an STD), and several Trypanosoma species which cause sleeping sickness Protista

12. Plasmodium

13. Giardia lamblia

14. African trypanosomiasis : symptoms in two stages haemolymphatic phase: fever, headaches, joint pains, and itching; severe swelling of lymph nodes; Winterbottom's sign, the tell-tale swollen lymph nodes along the back of the neck, may appear If untreated: anemia, endocrine, cardiac, and kidney dysfunctions neurological phase: parasite invades the central nervous system; term 'sleeping sickness' comes from these symptoms: confusion, reduced coordination, disruption of the sleep cycle with bouts of fatigue punctuated with manic periods  lead to daytime slumber and night-time insomnia Without treatment, invariably fatal; progressive mental deterioration leading to coma and death; damage caused in the neurological phase is irreversible Trypanosoma – Sleeping Sickness

15. The Origin and Early Diversification of Eukaryotes

16. Unique to Eukaryotes

17.  Membrane-bound nucleus  Endomembrane system  Mitochondria  Chloroplast  Cytoskeleton  9+2 flagella  Multiple chromosomes and linear DNA assoc. with proteins  Life cycles with mitosis, meiosis, and sex Unique to Eukaryotes

18. Trends Leading to Increased Complexity

21. The Endomembrane System

23. Endosymbiosis

24. Endomembrane System and Endosymbiosis

27. Leucoplast – (food cupboard) – stores starches Chromosplast – (paint can/food coloring) – gives flowers their colors Chloroplast – (solar panel) – converts light energy into chemical energy in the form of sugar and starch. Plastids

28. Existence of current endosymbiotic relationships Mitochondria and chloroplast size vs. prokaryotes Inner membranes of mito/chloro contain enzymes and transport systems like modern prokaryotes (ETC anyone?) Mito/chloro replicate similarly to binary fission Mito/chloro have single circular genome like prokaryotes Mito/chloro have t-RNA, ribosomes, and other “machinery” necessary to synthesize proteins Evidence for Endosymbiosis Which evidence is strongest? Which evidence is weakest?

29. Chimera

30. Cyanobacteria – ancestors of chloroplasts Alpha Proteobacteria – ancestors of mitochondria Eukaryotic Cell Organelle Ancestors

33. Protista!

34. Make a wet mount slide of the “pond” water (from your very own GCM ditch) using about 2 drops of “mucky” water Observe the protists in the water! Draw at least 6 diagrams total of protists you observe under 10X and 40X magnifications (3 in each?) Make your drawings as detailed as possible and label parts you think you can identify. Be sure to include the magnification under which you are observing. Survey of Protists!