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Published byMagnus Leo Patrick Modified over 9 years ago
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Assignment Strategies -N-C -CO-N-C -CO- H H-C-H H O-C-O HH H-C-H O-H -N-C -CO-N-C -CO- H H-C-H H O-C-O HH H-C-H O-H Homonuclear Two steps needed Scalar coupling within each amino acid Dipolar coupling between amino acids Heteronuclear Build up one atom at a time Scalar coupling within and between each amino acid; some experiments provide intra- and inter-residue peaks
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Pairs of Experiments Distinguishes Intra-residue from Inter-residue -N-C -CO-N-C -CO- H H-C-H H H HH H HNCA H(t 1 ), N(t 2 ), CA(t 3 ) 2 peaks- Intra + Inter Mixing from N C occurs over 1 bond to same C and over 2 bonds to adjacent C -N-C -CO-N-C -CO- H H-C-H H H HH H HN(CO)CA H(t 1 ), N(t 2 ), CA(t 3 ) 1 peak- Inter only Mixing from N C occurs ONLY over 2 bonds to adjacent C
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-N-C -CO-N-C -CO- H H-C-H H H HH H HNCACB H(t 1 ), N(t 2 ), CA and CB(t 3 ) 4 peaks- intra + inter Mixing from N C occurs over 1 bond to same C and over 2 bonds to adjacent C -N-C -CO-N-C -CO- H H-C-H H H HH H HN(CO)CACB H(t 1 ), N(t 2 ), CA and CB(t 3 ) 2 peaks- inter only Mixing from N C occurs ONLY over 2 bonds to adjacent C Pairs of Experiments Distinguishes Intra-residue from Inter-residue
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-N-C -CO-N-C -CO- H H-C-H H H HH H CBCANH CB and CA(t 1 ), N(t 2 ), H(t 3 ) Mixing from N C occurs over 1 bond to same C and over 2 bonds to adjacent C -N-C -CO-N-C -CO- H H-C-H H H HH H HNCACB H(t 1 ), N(t 2 ), CA and CB(t 3 ) Mixing from N C occurs over 1 bond to same C and over 2 bonds to adjacent C Equivalent Experiments Can be run in either direction
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