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Vijaya Ramachandran Fig S1 D C Supplementary Figures Invasion Apoptosis A Proliferation BxPC3 0 100 200 300 400 500 % of Viable Cells  AGR2(nM) 0 10 100.

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Presentation on theme: "Vijaya Ramachandran Fig S1 D C Supplementary Figures Invasion Apoptosis A Proliferation BxPC3 0 100 200 300 400 500 % of Viable Cells  AGR2(nM) 0 10 100."— Presentation transcript:

1 Vijaya Ramachandran Fig S1 D C Supplementary Figures Invasion Apoptosis A Proliferation BxPC3 0 100 200 300 400 500 % of Viable Cells  AGR2(nM) 0 10 100  0 100 200 300 400 500 600 % of Viable Cells  MiaPaCa-2  AGR2(nM) 0 10 100 B Migration 0 20 40 60 80 100 BxPC3   Migrated Cells per field AGR2(nM) 0 10 100 0 20 40 60 80   MiaPaCa-2 Migrated Cells per field AGR2(nM) 0 10 100 0 40 80 120   BxPC3 Invaded Cells per field AGR2(nM) 0 10 100 0 20 40 60 MiaPaCa-2   Invaded Cells per field AGR2(nM) 0 10 100

2 Vijaya Ramachandran Lysate+IgG Ab (Mouse) Lysate+AGR2 Ab (Mouse) 25kD 75kD 50kD 35kD CD59 – 30kD 20kD 15kD 10kD Lysate alone Marker B 25kD 75kD 50kD 35kD 100kD 250kD 150kD C4.4A – 50kD Lysate+IgG Ab (Mouse) Lysate+AGR2 Ab (Mouse) Lysate alone Marker A Lysate+IgG Ab (Mouse) Lysate+AGR2 Ab (Mouse) 25kD 75kD 50kD 35kD uPAR – 52kD 20kD 15kD 10kD Lysate alone Marker C Fig S2 WB

3 Vijaya RamachandranFig S2 25kD 75kD 50kD 35kD 100kD 250kD 150kD UPAR – 52kD 5nM Marker E 10nM 5nM 10nM  -Actin – 42kD SiCon SiUPAR 25kD 75kD 50kD 35kD 100kD 250kD 150kD C4.4A – 50kD F  -Actin – 42kD 5nM Marker 10nM 5nM 10nM SiCon SiC4.4A 25kD 75kD 50kD 35kD 100kD 250kD 150kD CD59 – 30kD D  -Actin – 42kD 5nM Marker 10nM 5nM 10nM SiCon SiCD59 WB

4 Vijaya Ramachandran C4.4A – 50kD Marker 25kD 75kD 50kD 35kD 100kD 250kD 150kD G. IP Fig S2 Marker BxPC-3 SU86.86 MPanc96 MiaPaCa-2 Panc-1 CFPac-1 AsPC-1 H. RT-PCR Blank C4.4A I. WB Marker BxPC-3 SU86.86 MPanc96 MiaPaCa-2 Panc-1 HPac-1 HPAF-2 CFPac-1 AsPC-1 C4.4A 25kD 75kD 50kD 35kD 100kD 250kD 150kD rProteins +AGR2 Ab rProteins +IgG Ab

5 BxPC-3 SiCont SiC44ASiCD59 SiUPAR 0 20 40 60 80 100 Viable Cells (% SiControl)  A Vijaya Ramachandran Fig S3 0 10 20 30 40 50 60 70 % of apoptotic cells   Gem(1uM) - + + - + + AGR2(100nm) - - + - - + BxPC-3 SiControlSiC4.4A D   Invasion Migration Proliferation Apoptosis  B SiCont SiC44ASiCD59 SiUPAR 0 50 100 150 Migratd Cells (% SiControl)  SiCont SiC44ASiCD59 SiUPAR 0 20 40 60 80 100 120 Migrated Cells (% SiControl + AGR2) + AGR2  SiCont SiC44ASiCD59 SiUPAR 0 20 40 60 80 100 120 Viable Cells (% SiControl + AGR2)  C SiCont SiC44ASiCD59 SiUPAR 0 20 40 60 80 100 120 Invaded Cells (% SiControl)  SiCont SiC44ASiCD59 SiUPAR 0 50 100 150 Invaded Cells (% SiControl + AGR2) Basal + AGR2

6 Vijaya Ramachandran Fig S4 BxPC-3 AGR2 - + + + + + + 0 50 150 250 350 450  SiControl SiITG-  6 SiITG-  1 SiITG-  4 SiLAM1SiLAM5  Viable Cells (% SiCon) B 

7 Marker Endo Recomb Commercial Ab Marker Endo Recomb 1A Marker Endo Recomb 1C Marker Endo Recomb 2B Marker Endo Recomb 8 Marker Endo Recomb 9B 10A11A12A 13A14B15B B. C4.4A mAbs Binding: Marker Endo Marker Endo Marker Endo Marker Endo Marker Endo Marker Endo Comm Ab 3A6A9B 10A15B 19 2025B28B29B30 A. AGR2 mAbs Binding: Vijaya Ramachandran Fig S5

8 Vijaya Ramachandran Fig S5 C. AGR2 mAbs Apoptosis Assay: D.C4.4A mAbs Apoptosis Assay: E. AGR2 - ELISA Binding Assay: G. Ab Purity: F. C4.4A - ELISA Binding Assay:

9 Vijaya Ramachandran Fig S6 A. Model 1-AsPc-1-Aggressive Model-Tumor Volume B. Model 2-CaPan-2-Stromal Model-Tumor Volume C. Model 3-CaPan-2-Regression Study-Tumor Volume Group Mice 123456 0 0.5 1.0 1.5 2.0 Control IgG Control IgG+Gem Combo IgG Combo IgG+Gem Weeks 7 Tumor Volume (Photons / cm 2 x 10 10 ) Sacrificed Treated 246810 0 2 4 6 8 50 556065 Control Ab AGR2 Ab C4.4A Ab Weeks Tumor Volume (Photons / cm 2 x 10 10 ) Group Mice No Treatment Treated Individual Mice No Treatment Treated Group Mice 123456789101112 0 2.0 4.0 6.0 8.0 1.0 1.2 Control Ab AGR2 Ab C4.4A Ab Combo Ab Weeks Tumor Volume (Photons / cm 2 x 10 10-11 ) Treated Individual Mice Treated Individual Mice Sacrificed Treated


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