1. Root nodule bacteria Tomoki Kawabata Maria Shimada

2. Abstract efficiently producing rhizobia Gives nutrients to the soil

3. Test 1 white clover seeds

4. Test 1 ( ２） Conditions ① Agar medium ② Murashige and Skoog medium ③ Murashige and Skoog medium with sucrose

5. Test 1 Result

6. Test 1 Consideration White clovers were not sterilized enough Murashige and Skoog medium were contaminated

7. Test 1 consideration White clover planted in Agar medium stopped growing two days after being planted. Agar media gradually lost their nutrition

8. Test 2 Study roots extension and the number of root nodules

9. Test 2 （１） Plants use the seeds of the white clover whose root extended

10. Test 2 ① HYPONeX ＋ water ② HYPONeX ＋ Mamezo ③ HYPONeX ＋ Root nodule bacteria extract (2) Consideration

11. Test 2

12. （３） Conditions Conditions were the same as Test 1

13. Test 2 Result/Consideration HYPONeX lowers the density of it There were no plants growing on the root nodule at all.

14. Test 3 relationship between extension of roots and root nodules I studied the extension of the root and its relationship with the number of plants growing on the root nodule

15. Test 3 （１） Plant Use the White clover seeds

16. Test3 （２） Conditions ① water ② MAMEZO solutions ③ Root nodule bacteria extract

17. Test 3 （３） Conditions Conditions were the same as test 1

18. Test 3 Result

19. 実験Ⅲの結果

20. Test 3 Consideration Azospirillum bacteria in Mamezo synthesized Auxin promoted the extension of ② roots

21. Test 3 Consideration The nodule growth The nodules stopped growing and adding the root nodules

22. Thank you for listening