1. More efficient paper device using PNIPAM 20091810 김영완 20091855 장욱일 20101899 장수영 20101885 이원진

2. Microfluidic paper-based device technology Paper-based microfluidics -> burgeoning research field with its beginning in 2007 -> variety of applications ; health diagnostics, environmental monitoring, food quality test, etc. -> low cost, easy-to-use, disposable, equipment-free On demand device Ready to use device -> Blank microfluidic platforms without pre-deposited reagents -> Integrate indication reagents into detection zone We will use hydrogel for storage of reagents in ready to use device

3. Microfluidic paper-based device technology Current limitation of paper-based microfluidic device Sample retention and low efficiency of sample delivery within the device -> More efficient sample delivery is needed -> precise control of lateral flow in paper To solve this problem, we will use hydrogel X. Li, et al., Biomicrofluidics(2012)

4. Microfluidic paper-based device technology Manufacturing cost A. K. Yetisen, et al., Lab on a Chip(2013) + hydrogel

5. Microfluidic paper-based device technology Quantitative goal Increase the efficiency of reagents delivery to more than 50 % using hydrogel Add engineering analysis of flow through the channel and develop channel

6. ELISA (Enzyme-Linked Immunosorbent Assay) : Protein antigen-antibody reaction ABO typing : ABO typing kit Application of paper-based device

7. Plan \202,000+\12,000+\12,000+\21,000 = \247,000 PNIPAM storage and flow analysis test with water