1. White Blood Cell Differential Count

2. Objectives
To able to identify the different types of leucocytes under the microscope
To practice the procedure for differential leucocytes counting
To know normal values for the different white cell count
To know the importance of DLC in the diagnosis of disease processes.

3. Definition
The relative percentage of each type of white blood cells in peripheral blood.
This experiment is a part of blood routine test.

4. Microscopic exam
10× (low fold): overall smear quality, agglutination or parasites
100× (oil Len): WBC Diff, RBC morphology

5. Observing direction:
Observe one field and record the number of WBC according to the different type then turn to another field in the snake-liked direction
*avoid repeat or miss some cells

6. Morphology of WBC in peripheral blood

7. normal peripheral blood smear

9. White blood cells
There are three types of granulocyte named according to their staining characteristics in blood films. They are neutrophils,eosinophils and basophils.
Agranular cells are divided into lymphocytes and monocytes.

10. Neutrophils 62% of total WBCs Diameter: 10-12 microns
Cytoplasm: very pale blue
Granules: staining (purple)
Nucleus: dark purple blue 2-5 lobes

11. Neutrophils .
neutrophilic because they owe their colour to uptake of both
the acidic and the basic components of the stain

13. Eosinophil Diameter: 13-14 microns
Cytoplasm : full of orange-red coarse granules
Nucleus:
blue 2 lobes like a pair of glass
2.3%

15. Basophil Diameter: 14-15 Cytoplasm:
Granules: dark blue –black obscure nucleus
Nucleus: blue
0.4%

17. Lymphocyte Diameter: small 7-9 large 12-16
Cytoplasm : rim, clear, pale blue
Nucleus: purple red ,oval indented
30%

18. Normal lymphocytes Lymphocytes are the smallest WBC.
They have large condensed nucleus, with a scanty pale blue cytoplasm.

20. Monocyte Diameter: 16-18(are the largest normal blood cells)
Cytoplasm: The cytoplasm is abundant, sky blue in colour.
Some have vacuoles in the cytoplasm.
Nucleus: purple, large kidney shaped and slightly indented.
5.8%