1. Copyright © by Holt, Rinehart and Winston. All rights reserved. ResourcesChapter menu Recombinant DNA Cloning Vectors –Researchers use restriction enzymes to insert DNA fragments into vectors –The resulting DNA from two different organisms is called recombinant DNA Plasmids –Closed loops of DNA found in bacteria –Can be separated from the bacterial chromosome and replicated on their own within the cell Ch 9-4

2. Copyright © by Holt, Rinehart and Winston. All rights reserved. ResourcesChapter menu Cloning Vectors and Plasmids

3. Copyright © by Holt, Rinehart and Winston. All rights reserved. ResourcesChapter menu Recombinant DNA Transgenic –An organism with one or more genes from another organism inserted into its genome Gene Knockout –Genes that have been purposely “turned off” –Disrupting the function of the gene –Used in mice to study gene function and diseases

4. Copyright © by Holt, Rinehart and Winston. All rights reserved. ResourcesChapter menu Cloning –a nucleus from a body cell of one individual is introduced into an egg cell (without its nucleus) from another individual –An organism identical to the nucleus donor results