1. Chapter 12 DNA and RNA *This presentation contains copyrighted material.

2. DNA and Crime Scene Investigations –Many violent crimes go unsolved For lack of enough evidence –If biological fluids are left at a crime scene DNA can be isolated from them

3. –DNA fingerprinting is a set of laboratory procedures That determines with near certainty whether two samples of DNA are from the same individual That has provided a powerful tool for crime scene investigators Investigator at one of the crime scenes (above), Narborough, England (left)

4. BACTERIAL PLASMIDS AND GENE CLONING 12.1 Plasmids are used to customize bacteria: An overview –Gene cloning is one application of DNA technology Methods for studying and manipulating genetic material

5. –Researchers can insert desired genes into plasmids, creating recombinant DNA And insert those plasmids into bacteria Bacterium Bacterial chromosome Plasmid 1 isolated 3 Gene inserted into plasmid 2 DNA isolated Cell containing gene of interest DNA Gene of interest Recombinant DNA (plasmid) 4 Plasmid put into bacterial cell Recombinant bacterium 5 Cell multiplies with gene of interest Copies of protein Copies of gene Clone of cells Gene for pest resistance inserted into plants Gene used to alter bacteria for cleaning up toxic waste Protein used to dissolve blood clots in heart attack therapy Protein used to make snow form at higher temperature Figure 12.1

6. –If the recombinant bacteria multiply into a clone The foreign genes are also copied

7. Understanding Genetics Well, then…we need to understand the chemical makeup of the gene I’d like to better understand genetics…

8. Understanding Genetics Yeah, DNA can store genetic information, and transmit it to the next generation! Sweet! Genes are made of DNA…

9. Understanding Genetics Far out, maybe we’ll find out how it decides traits and replicates itself, too! Let’s study this DNA and figure out how it carries information

10. Understanding Genetics Dude – DNA can make a copy of itself – that’s called replication! Replicates? What on earth does that mean?

11. History and Structure In 1953, two scientists, James Watson & Francis Crick, built model of DNA out of tin & wire –Looked like a twisted ladder –Called the shape a Double Helix (2 twisting strands)

13. Structure Sides of ladder are sugar & phosphate groups Rungs of ladder are nitrogen bases joined together by H bonds

16. DNA Structure

17. DNA = deoxyribonucleic acid –Long molecules of nucleotides linked together like a chain

20. Structure Each nucleotide is made of 3 parts: –Phosphate Group –5-Carbon Sugar (deoxyribose) –Nitrogen Base

21. NUCLEOTIDE!!!!

22. Structure 5-C sugar & phosphate group always the same for each nucleotide in DNA –Form sides of ladder

23. Structure There are 4 different nitrogen bases: –Adenine –Guanine –Thymine –Cytosine

26. Structure Adenine & Guanine classified as Purines –Because they are larger molecules with double rings of Carbon & Nitrogen

27. Structure Cytosine & Thymine classified as Pyrimidines –Because they are made of a single ring of Carbon & Nitrogen

28. Note the single and double rings. What else do you notice?

29. BASE PAIRING Since A always pairs with T (A-T) and C always pairs with G (C-G), DNA strands considered complementary to each other –They match –Think of the color wheel!

30. Are the same in every organism The nucleotides are in a DIFFERENT order for each organism

31. DNA is found in the nucleus of cells. - contain both DNA and protein tightly packed together to form a substance called chromatin.

32. An area of coded DNA on a given chromosome that gives a certain trait. Traits such as height, eye and hair color is coded in your genes.

33. To analyze chromosomes, cell biologists PHOTOGRAPH cells in MITOSIS. Next, they cut out the chromosomes and group them in pairs. This is called a KARYOTYPE

34. Replication is the process of copying DNA During replication each strand serves as a pattern to make a new DNA molecule

35. Enzymes (DNA polymerase) break hydrogen bonds between nitrogen bases Molecule unzips Unzipped molecule base pairs with free nucleotides

36. Two copies of the organism’s genetic information that can be passed during mitosis or meiosis

37. Replication Summary In DNA replication, enzymes work to unzip & separate the double helix. Complementary bases are added to the open strands. Each new double helix is made of one old DNA strand & one new strand. http://nobelprize.org/educational_games/medicine/dna_double_helix/

38. 12-3: Protein Synthesis

39. RNA is… Single stranded Contains ribose Contains the base uracil instead of thymine

40. Three types: –Messenger (mRNA) Carry copies of instructions for assembling amino acids into proteins. –Ribosomal (rRNA) Along with proteins, rRNA makes up ribosomes where proteins are assembled. –Transfer (tRNA) Transfers amino acids to the ribosome as it is specified by coded messages in mRNA.

43. Process of making an RNA copy of a DNA strand Results in the formation of a single- stranded RNA molecule

44. mRNA carries the information for making proteins to the ribosomes Since directions for making proteins are in the nucleus the mRNA has to take the info to the cytoplasm where the ribosomes can make proteins!

45. DNA molecule unzips Free RNA nucleotides pair with complementary DNA strands mRNA breaks away and the DNA strand rejoins

46. Proteins are made by joining amino acids into long chains called polypeptides. Each polypeptide contains any combination of the 20 amino acids. –The properties of proteins are determined by the order of each of these different amino acids.

47. Each set of three nitrogen bases representing an amino acid is known as a codon—triplet code 64 combinations possible; 61 code for amino acids and 3 code for stop signals

50. The process of converting information in a sequence of nitrogen bases in mRNA into a sequence of amino acids that make up a protein Decoding the message

51. tRNA brings amino acids to the ribosomes so they can be assembled into proteins

55. So you may be thinking…. Why do we need proteins? Proteins: –Most are enzymes that code for…. –Production of a pigment to control the color of a flower –Production of red blood cell surface antigen (determines blood type) –Regulate the rate and pattern of growth throughout an organism (controls size and shape)

56. Mutations Sometimes cells make mistakes when copying their own DNA…these mistakes are called MUTATIONS! –Changes in the genetic material of the cell. Many mutations are not harmful, some even increase genetic variation in a species! Mutations that are harmful can cause genetic disorders and sometimes cancer.

57. Types of Mutations Single base-pair substitution Deletion or insertion of one or more base pairs Major alteration in the structure of a chromosome. May or may not bring about a change in a phenotype. WHY?

58. Mutations-classified by location Cells –Somatic: occur in any cell in the body except gametes (Localized cell death, altered cellular function, or tumors) –Sex-cells: occur in gametes (creates genetic diversity and/or disease) Chromosome…in all of the cells! –Autosomal: occur within genes located on the autosomes (chromosome pairs 1-22) –X-linked: occur on the X chromosome

59. Mutations: classified by type of change POINT (changes base pair) If there is not a change in AA, then it is a silent mutation. Missense mutation: Change of one nucleotide, may change the AA that the codon called for. Nonsense mutation: Triplet will be changed into a stop codon, resulting in a termination of translation. FRAMESHIFT Addition or deletion of a single letter, which causes all of the rest of the letters to be “MOVED” by one space.