1. pGLO followup and term 4!!!

2. Announcements:  Before April Break you must schedule a 5 minute conference with me after school or at 7:45. List is on the board. This is a HW assignment to set goals for term 4!  pGLO lab is due Friday 4/11  Extra help is available next Tuesday from my senior helpers! You must have a draft to work with!

3. Questions to consider for introduction:  What do the terms “bioengineering” and “cloning” mean?  Where is the gene originally from?  What does the plasmid contain?  How will we get the plasmid into the bacteria?  What will activate the production of GFP in bacteria?

4. Peer Review of Introduction  Advanced: Discusses concepts and links to the specific goal in this lab!!!  Proficient: Discusses bioengineering, cloning, plasmids and heat shock. Links them to eachother but does not tie to the purpose of bioengineering in this lab.  Needs Improvement : Describes most of the concepts but does not link them to eachother…or…misses concepts  Give your partner an assessment and write a sentence or two to explain why!

5. Collection of Data  For the purposes of this lab, we are going to call transformation efficiency the number of colonies produced!  You need a table to show the results on all four of your plates as well as a graphical representation (picture, etc).  For class data, we will look at our “experiment within an experiment”  Temperature of “cloning procedure”  Transformation efficiency  How could you choose to display these results?

6. Conclusions:  What did we learn about the plasmid system we used based on our results?  What were some reasons for variation within the data…is there anything we could modify?  What else could apply our new found knowledge of bioengineering to?  Use the guiding questions on your lab sheet but don’t let them limit you!!!