Presentation is loading. Please wait.

Presentation is loading. Please wait.

0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a.

Published byHubert Welch Modified over 9 years ago

Similar presentations

Presentation on theme: "0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a."— Presentation transcript:

1 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a

2 MCF-7 Figure S1b * **

3 - - + + - - - + - + - +Paclitaxel 100 nM Bad siRNA Scrambled siRNA - - - - + + Bad Actin IP: Bcl-2 IB: Bcl-2 Input IB: Bcl-2 Bcl-2 Actin IP: Bcl-2 IB: Bim BimEL BimL BimS MCF-7 Figure S1c

4 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel Cleaved Caspase-3 T47D Figure S1d

5 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel Cleaved Caspase-3 MDA-MB-468 Figure S1e

6 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel Cleaved Caspase-3 BT20 Figure S1f

7 IP: Bcl-xL IB: Bcl-xL Bcl-xL Bim EL Bim L Bim S IP: Bcl-xL IB: Bim Mcl-1 Paclitaxel 100 nM 0 4 8 12 [h] Bim EL Bim L Bim S IP: Mcl-1 IB: Bim Paclitaxel 100 nM 0 4 8 12 [h] IP: Mcl-1 IB: Mcl-1 Figure S2a MCF-7

8 Figure S2b Pre-IPPost-IP BimEL BimL BimS HM LMS-100 Untreated Taxol 12 h CoxIV BiP/Grp78 BimEL BimL BimS 1 0.43 Actin Ratio Paclitaxel 100 nM - + Pre-IPPost-IP Actin BimEL BimL BimS Untreated 1 1.39Ratio HM LMS-100 MCF-7

9 - + - + Paclitaxel 100 nM MCF-7 MCF-7 Bcl-2+ FLAG-Bcl-2 Bcl-2 FLAG-Bcl-2 BimEL BimL BimS IP: FLAG IB: Bcl-2 Input IB: Bcl-2 IP: FLAG IB: Bim Bmf IP: FLAG IB: Bmf Input IB: Bmf BimEL BimL BimS Input IB: Bim Actin IP: FLAG IB: Bax IP: FLAG IB: Puma * IP: FLAG IB: Bak IP: FLAG IB: Bad - + - + Paclitaxel 100 nM MCF-7 MCF-7 Bcl-2+ Bad Puma Bax Bak Figure S2c

10 Figure S2d IP: Bcl-2 IB: Bax Bax IP: Bcl-2 IB: Bak Paclitaxel 100 nM - + IP: Bcl-xL IB: Bax IP: Bcl-xL IB: Bak Paclitaxel 100 nM - + IP: Mcl-1 IB: Bax IP: Mcl-1 IB: Bak Paclitaxel 100 nM - + Bak Bax Bak Bax Bak MCF-7

11 0 4 8 12 24 48 0 4 8 12 24 48 (hr) MitoCyto Cytochrome c CoxIV T47D Figure S3a

12 0 4 8 12 24 48 0 4 8 12 24 48 (hr) MitoCyto MDA-MB-468 Figure S3b Cytochrome c CoxIV

13 0 4 8 12 24 48 0 4 8 12 24 48 (hr) MitoCyto BT20 Figure S3c Cytochrome c CoxIV

14 0 4 8 12 24 48 (hr) Bax Bak IP: Bax 6a7 IB: Bax N20 IP: Bak Ab-2 IB: Bak G23 100 nM Paclitaxel T47D Input IB: Bax N20 Input IB: Bak G23 Bax Bak Actin Figure S4a

15 0 4 8 12 24 48 (hr) Bax Bak IP: Bax 6a7 IB: Bax N20 IP: Bak Ab-2 IB: Bak G23 100 nM Paclitaxel MDA-MB-468 Input IB: Bax N20 Input IB: Bak G23 Bax Bak Actin Figure S4b

16 0 4 8 12 24 48 (hr) Bax Bak IP: Bax 6a7 IB: Bax N20 IP: Bak Ab-2 IB: Bak G23 100 nM Paclitaxel BT20 Input IB: Bax N20 Input IB: Bak G23 Bax Bak Actin Figure S4c

17 Puma Actin - - + + - - - + - + - +Paclitaxel 100 nM Puma siRNA Scrambled siRNA - - - - + + MDA-MB-468 Figure S5a

18 Puma Actin - - + + - - - + - + - +Paclitaxel 100 nM Puma siRNA Scrambled siRNA - - - - + + BT20 Figure S5b

19 Puma Actin - - + + - - - + - + - +Paclitaxel 100 nM Puma siRNA Scrambled siRNA - - - - + + MCF-7 Figure S5c

Download ppt "0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a."

Similar presentations

Jungwirth et al. Suppl. Fig. 1 A B. Jungwirth et al. Suppl. Fig. 2 HCT-116  -actin Bim EL Bak Bax - + KP46 A549 - + A C KP46 (µM) 0 2.5 10 2.5 10 4 hours.

Jungwirth et al. Suppl. Fig. 1 A B. Jungwirth et al. Suppl. Fig. 2 HCT-116  -actin Bim EL Bak Bax - + KP46 A A C KP46 (µM) hours.
ABAB p53 caffeine doxo - - - 24h 48h - + + - + Casp8 Casp10 FADD FLIP Bcl-2 BIM BID PUMA Bax Bak XIAP cIAP-1 cIAP-2 GAPDH MFI untreated 3,3x10 3 MFI doxo.

ABAB p53 caffeine doxo h 48h Casp8 Casp10 FADD FLIP Bcl-2 BIM BID PUMA Bax Bak XIAP cIAP-1 cIAP-2 GAPDH MFI untreated 3,3x10 3 MFI doxo.
Next Steps in Literature Mining Marti Hearst UC Berkeley ASIST 2003 Literature Mining Panel.

Next Steps in Literature Mining Marti Hearst UC Berkeley ASIST 2003 Literature Mining Panel.
Supplemental Information for: METABOLISM OF RAMELTEON IN HUMAN LIVER MICROSOMES AND CORRELATION WITH THE EFFECT OF FLUVOXAMINE ON RAMELTEON PHARMACOKINETICS.

Supplemental Information for: METABOLISM OF RAMELTEON IN HUMAN LIVER MICROSOMES AND CORRELATION WITH THE EFFECT OF FLUVOXAMINE ON RAMELTEON PHARMACOKINETICS.
30 patient drug sensitivity data Figure S1. Figure S2A Myeloid regulator enrichment in AML.

30 patient drug sensitivity data Figure S1. Figure S2A Myeloid regulator enrichment in AML.
Figure 1 B D DOX ( μ M) C Cell Proliferation (% Con) MCF-7MCF-7/DOX 0 0.1 1 DOX ( μ M) 0.03% 38.79% 0.15% 0.10% 0.20% 0.05% 0 20 40 60 80 100 120 00.110.

Figure 1 B D DOX ( μ M) C Cell Proliferation (% Con) MCF-7MCF-7/DOX DOX ( μ M) 0.03% 38.79% 0.15% 0.10% 0.20% 0.05%
Supplemental Figure S1 A B MDA-MB-231 MCF-7 BCL-2MDA-MB-231 BCL-2 MCF-7 Bcl-2 Actin Bcl-2 Actin.

Supplemental Figure S1 A B MDA-MB-231 MCF-7 BCL-2MDA-MB-231 BCL-2 MCF-7 Bcl-2 Actin Bcl-2 Actin.
Cell death vs Cell life. Characteristic morphologic features of apoptosis Extr signal Intr signal nucleus DNA fragmentation (formation of nucleosomal.

Cell death vs Cell life. Characteristic morphologic features of apoptosis Extr signal Intr signal nucleus DNA fragmentation (formation of nucleosomal.
Constituent protein characteristics Constituent detected peptide characteristics Figure S1A.

Constituent protein characteristics Constituent detected peptide characteristics Figure S1A.
Figure S1 HA-FOXO3 wt C terminus ab HA-FOXO3 wt HA ab untransfected C terminus ab untransfected HA ab 5.9 6.7 6.4.

Figure S1 HA-FOXO3 wt C terminus ab HA-FOXO3 wt HA ab untransfected C terminus ab untransfected HA ab
FIGURE S1 S1.c Scr. iPARG 30 nM H2O2 PAR iPARP-1 60 nM DAPI S1.a S1.b

FIGURE S1 S1.c Scr. iPARG 30 nM H2O2 PAR iPARP-1 60 nM DAPI S1.a S1.b
Volume 24, Issue 1, Pages (July 2013)

Volume 24, Issue 1, Pages (July 2013)
Progesterone receptor

Progesterone receptor
Proapoptotic BAX and BAK control multiple initiator caspases

Proapoptotic BAX and BAK control multiple initiator caspases
Mitochondrial Ferritin Protects Hydrogen Peroxide-Induced Neuronal Cell Damage Guofen Gao 1 ;Nan Zhang 1 ;Yue-Qi Wang 1 ;Qiong Wu 1 ;Peng Yu 1 ;Zhen-Hua.

Mitochondrial Ferritin Protects Hydrogen Peroxide-Induced Neuronal Cell Damage Guofen Gao 1 ;Nan Zhang 1 ;Yue-Qi Wang 1 ;Qiong Wu 1 ;Peng Yu 1 ;Zhen-Hua.
Supplementary Figure S1.

Supplementary Figure S1.
Restoring cancer's death sentence

Restoring cancer's death sentence
Supplemental Figures Supplemental Figure 1.

Supplemental Figures Supplemental Figure 1.
Volume 129, Issue 1, Pages (July 2005)

Volume 129, Issue 1, Pages (July 2005)
Model for mitosis‐induced apoptosis.

Model for mitosis‐induced apoptosis.

Similar presentations

Ads by Google