1. P50 GM073197 JCIMPT NMR Team ____________________ Dr. Reto Horst Dr. Pedro Serrano Dr. Pawel Stanczak __________________________________________________ Dr. Kurt Wüthrich

3. Screening of New Detergents Dr. Reto Horst Dr. Qinhai Zhang Michael Geralt Financial Support through JCIMPT (PI Dr. Ray Stevens) by the NIH Roadmap Initiative and through JCSG (PI Dr. Ian Wilson) by the NIH PSI.

4. 600 μL5 μL Gilson Liquid Sample Handler Bruker Automated Sample Changer 1 mm diameter capillary tube vs 5 mm diameter tube NMR Screening Setup

5. [ 15 N, 1 H]-TROSY spectra of refolded [ 2 H, 15 N]-labeled OmpW in: DHPCFos30

6. NMR BUFFER: 20 mM Na Phosphate pH = 6.8 100 mM NaCl 0.3 % (v/v) NaN 3 10 % (v/v) D 2 O 2 % (w/w) DHPC NMR BUFFER: 5 mM Na Phosphate pH = 6.8 10 mM NaCl 0.3 % (v/v) NaN 3 10 % (v/v) D 2 O ≤1 % (w/w) Detergent Reconstitution of OmpX from inclusion bodies Detergent DHPCNew Detergents

7. NMR Sample Preparation of OmpX

8. Micro-scale 1D 1 H NMR Measurement of Detergent Concentration

9. NMR Sample Preparation of OmpX

10. Monitoring Detergent Concentration Using Micro-coil 1D 1 H NMR

12. Impact of Protein Concentration

13. 1.7 % Fos-10 [OmpX] = 0.84 mM 3.3 % Fos-10 [OmpX] = 1.04 mM 28.5 % Fos-10 [OmpX] = 0.77 mM [ 15 N, 1 H]-TROSY-NMR

14. 1.7 % Fos-10 [OmpX] = 0.84 mM 3.3 % Fos-10 [OmpX] = 1.04 mM 28.5 % Fos-10 [OmpX] = 0.77 mM [ 15 N, 1 H]-TROSY-NMR, cross-sections

15. 1.7 % Fos-10 [OmpX] = 0.84 mM 3.3 % Fos-10 [OmpX] = 1.04 mM 28.5 % Fos-10 [OmpX] = 0.77 mM [ 15 N, 1 H]-TROSY-NMR, cross-sections