1. Angiostatin: A Novel Angiogenesis Inhibitor That Mediates the Suppression of Metastases by a Lewis Lung Carcinoma Michael S. O’Reilly, Lars Holmgren, Yuen Shing, Catherine Chen, Rosallnd A. Rosenthal, Marsha Moses, William S. Lane, Yihai Cao, E. Helene Sage, and Judah Folkman Cell, Vol. 79, 315-328, October 21, 1994

2. Lung carcinoma implanted subcutaneously into mice. Allow to grow to 1500 mm 3 Removed (or not) and wait 15 days Look for metastases to lung

5. Same experiment, but now treat with serum from tumor-bearing animal

6. Same experiment, but now treat with serum or urine from tumor-bearing animal

11. Named this protein Angiostatin Surprise! It’s a fragment of Plasminogen

12. Named this protein Angiostatin Surprise! It’s a fragment of Plasminogen