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Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 1 X-Ray Facility Overview IMB X-Ray Facility (IMB XRF) 410-413 Kasha Laboratory www.sb.fsu.edu/~xray.

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Presentation on theme: "Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 1 X-Ray Facility Overview IMB X-Ray Facility (IMB XRF) 410-413 Kasha Laboratory www.sb.fsu.edu/~xray."— Presentation transcript:

1 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 1 X-Ray Facility Overview IMB X-Ray Facility (IMB XRF) 410-413 Kasha Laboratory www.sb.fsu.edu/~xray 850-644-6448

2 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 2 IMB X-Ray Facility (XRF)  IMB: Inter-disciplinary Institute  Structural Biology & Computational Biophysics  XRF: Core Facility [Est. 1993]  Open and Free Access  Safety & On-site Training Required

3 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 3 What XRF Can Do For You? CrystalDiffraction e - Density Structure Images courtesy of Michael Chapman Lab XRF can help you in Steps

4 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 4 X-Ray Generator & Mirror  Rigaku RU-H2R  Cu Rotating Anode  Brilliant Beam  Osmic Confocal Mirror  Wavelength Purity  High Flux www.rigaku.com

5 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 5 X-Ray Detectors Imaging Plate & CCD  Rigaku R-Axis IIc  Two 19cm 2 IPs  8 minute Read-out  MarCCD165  Single CCD  3 s Read-out

6 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 6 X-Ray (Cryo) Data Collection  MarCCD/CrystalClear  GUI  Automated  Analog»Digital  Oxford CryoSystem  Collect at 100°K  Protects Crystals  Sharper eDensity www.oxfordcryosystems.co.uk

7 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 7 CryoCrystallography  Flash Cool in Stream For Immediate Collection Ease of Handling  Flash Cool in Liquid For Storage For Shipping & Transport Flash Cool in Stream Flash Cool in Liquid

8 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 8 Amenities & Accessories  Crystal Room  Cold Room  Variable T Incubator  Digital Imaging  Synchrotron Time  Microscopes  Cryo Tools  Computer Licenses  3D Graphics  Sample Tools

9 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 9 Crystal & Data Services  Screening & Crystallization  Data Collection Strategy  Crystal Storage & Transport  Synchrotron Time & Usage  Data Processing & Visualization  Data Interpretation  Data Storage & Extraction

10 Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 10 XRF-Contact Thayumanasamy “Soma”sundaram 414 Institute of Molecular Biophysics Florida State University Tallahassee, FL 32306-4380 Phone: 850-644-6448 | Fax: 850-644-7244 E-mail: tsomasundaram@fsu.edutsomasundaram@fsu.edu Web: www.sb.fsu.edu/~xraywww.sb.fsu.edu/~xray

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