1. CASIROZ Fall Meeting Cambridge 2004 Participant group 2 Manuela C. Blumenröther W. Oßwald Forest Phytopathology, TU Munich

2. Seasonal pattern of carbohydrates 2003 Higher glucose and fructose concentrations in 2xO 3 sun leaves at most time points mean values per branch ± SE

3. Seasonal pattern of carbohydrates 2003 Lower sucrose and starch concentrations in 2xO 3 sun and shade leaves at most time points mean values per branch ± SE

4. Diurnal pattern of carbohydrates July 2003: Total soluble sugars & starch Carbohydrates used for repair in 2 x O 3 trees Significantly reduced starch concentrations in 2 x O 3 sun leaves at midday

5. Specific activity of RubisCO and PEPC [nkat*mg protein -1 ], June 2003; mean values ± SD no clearly visible effect of ozone on enzyme activity of RubisCO and PEPC

6. Parallel assessment of photosynthesis and carbohydrate content (August 03 & May 04) Photosynthesis Starch 2 x O 3 1 x O 3 2 x O 3 1 x O 3

7. Carbohydrates in relation to CO 2 uptake (parallel assessment August 2003) Glucose Fructose

8. Carbohydrates in relation to CO 2 uptake (parallel assessment August 2003) Sucrose Starch

9. Carbohydrates in relation to CO 2 uptake (August 2003) Sugar concentration [mg *g DW -1 ] A max [µmol m -2 s -1 ] Glucose Starch Fructose Sucrose

10. Carbohydrates in relation to CO 2 uptake (August 2003)

12. Sucrose in relation to CO 2 uptake (August 03 vs. May 04) CO 2 uptake [µmol m -2 s -1 ] Sucrose [mg*g DW -1 ]

13. Sucrose in relation to CO 2 uptake (August 03 vs. May 04) Sucrose [mg*g DW -1 ] CO 2 uptake [µmol m -2 s -1 ] August 03 1 x O 3 2 x O 3

14. Sucrose in relation to CO 2 uptake (August 03 vs. May 04) Sucrose [mg*g DW -1 ] CO 2 uptake [µmol m -2 s -1 ] May 04 1 x O 3 2 x O 3

15. Sucrose in relation to CO 2 uptake (August 03 vs. May 04) CO 2 uptake [µmol m -2 s -1 ] Sucrose [mg*g DW -1 ] August 03May 04 shade sun

16. Sucrose in relation to CO 2 uptake (August 03 vs. May 04) CO 2 uptake [µmol m -2 s -1 ] Sucrose [mg*g DW -1 ]

17. Starch pattern in sun leaves; October 2003 1 x O 3 2 x O 3

18. Thank you!!!

19. Seasonal pattern of carbohydrates in young trees; MV per treatment ± SE