1. Microscope Basics

2. 1.Ocular lens (Eyepiece)

3. 2.Body Tube

4. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION.

5. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X

6. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place

7. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place 6.DIAPHRAGM Regulates the amount of LIGHT on the specimen

8. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place 6.DIAPHRAGM Regulates the amount of LIGHT on the specimen 7.LIGHT SOURCE Projects light UPWARDS through the diaphragm, the SPECIMEN, and the LENSES

9. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place 6.DIAPHRAGM Regulates the amount of LIGHT on the specimen 7.LIGHT SOURCE Projects light UPWARDS through the diaphragm, the SPECIMEN, and the LENSES 8.BASE Supports the MICROSCOPE

10. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place 6.DIAPHRAGM Regulates the amount of LIGHT on the specimen 7.LIGHT SOURCE Projects light UPWARDS through the diaphragm, the SPECIMEN, and the LENSES 8.BASE Supports the MICROSCOPE 9.FINE ADJUSTMENT KNOB Moves the stage slightly to SHARPEN the image

11. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place 6.DIAPHRAGM Regulates the amount of LIGHT on the specimen 7.LIGHT SOURCE Projects light UPWARDS through the diaphragm, the SPECIMEN, and the LENSES 8.BASE Supports the MICROSCOPE 9.FINE ADJUSTMENT KNOB Moves the stage slightly to SHARPEN the image 10.COARSE ADJUSTMENT KNOB Moves the stage up and down for FOCUSING

12. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place 6.DIAPHRAGM Regulates the amount of LIGHT on the specimen 7.LIGHT SOURCE Projects light UPWARDS through the diaphragm, the SPECIMEN, and the LENSES 8.BASE Supports the MICROSCOPE 9.FINE ADJUSTMENT KNOB Moves the stage slightly to SHARPEN the image 10.COARSE ADJUSTMENT KNOB Moves the stage up and down for FOCUSING 11.STAGE Supports the SLIDE being viewed

13. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place 6.DIAPHRAGM Regulates the amount of LIGHT on the specimen 7.LIGHT SOURCE Projects light UPWARDS through the diaphragm, the SPECIMEN, and the LENSES 8.BASE Supports the MICROSCOPE 9.FINE ADJUSTMENT KNOB Moves the stage slightly to SHARPEN the image 10.COARSE ADJUSTMENT KNOB Moves the stage up and down for FOCUSING 11.STAGE Supports the SLIDE being viewed 12.ARM Used to SUPPORT the microscope when carried

14. 1.Ocular lens (Eyepiece) 2.Body Tube 3.Nosepiece Holds the HIGH- and LOW- power objective LENSES; can be rotated to change MAGNIFICATION. 4.OBJECTIVE LENSES Magnification ranges from 10 X to 40 X 5.STAGE CLIPS HOLD the slide in place 6.DIAPHRAGM Regulates the amount of LIGHT on the specimen 7.LIGHT SOURCE Projects light UPWARDS through the diaphragm, the SPECIMEN, and the LENSES 8.BASE Supports the MICROSCOPE 9.FINE ADJUSTMENT KNOB Moves the stage slightly to SHARPEN the image 10.COARSE ADJUSTMENT KNOB Moves the stage up and down for FOCUSING 11.STAGE Supports the SLIDE being viewed 12.ARM Used to SUPPORT the microscope when carried

15. What happens as the power of magnification increases? Power = 10 x 4 = 40 Power = 10 x 10 = 100Power = 10 x 40 = 400

16. Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

17. Let’s give it a try... 1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 4 – When you are done, turn off the microscope and put up the slides you used. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!

18. How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. You do not need to use the stage clips when viewing wet-mount slides! 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly

19. Onion Cell Reflection Write a paragraph answering these questions fluently. – Why do we use a microscope? – What did you learn about using a microscope today? – What things did you find difficult doing in the lab today? – Why do we stain cells with iodine? – What is the difference between animal cells and plant cells?

20. Cheek Cell Onion Cell