1. Sadia Tahir MARINE BIOTECHNOLOGY (FAB-369) Credit Hrs 3(2-1)

2. Course Contents:  Introduction to Marine Biology  Introduction and Significance  Major Aquatic Ecosystems (estuaries, coral reefs, intertidal, pelagic, benthic zones)  Importance of marine life in medical biotechnology (treatment of cancer and nervous system Disorders)  Marine resources (fishing, salt, water, underwater mining, energy, petroleum products)  Functional Foods: Definition, Classification, Design, Marine Products as Functional Foods  Seafood Proteins  Functional Properties  Protein Supplements  Fish Protein

3.  Powders, Seafood Products of Consumer Interest, Collagen, Gelatin  Seafood Proteins: Nutritional Value, Bioactive Peptides  Marine and Cold-Adapted Enzymes  Polyunsaturated Fatty Acids and Their Therapeutic Functions  Marine Lipids, Nutritional Value, Omega 3 Oils from Marine Fish  Commercial Significance  Seafood Processing Wastes: Chitin, Chitosan, and Other Compounds  Carotenoids: Marine Sources, Functional Roles, Dietary Carotenoids, Commercialization  Marine Sources of Vitamins and Minerals.  Seaweed: Nutritional Value, Bioactive Properties, Industrial Uses, Commercial Products  Seaweed Hydrocolloids: Agar, Alginate, Carrageenan  Cosmetics, Drugs and Pharmaceuticals from Marine Sources: Marine Secondary Metabolites, Marine herbs, minerals use in synthesis of cosmetics products, Marine Drugs in Use, Potential Marine bioactive Compounds, Global Interests and Commercial Status

4.  Marine Micro & Macro Algae: Components, Cultivation, Uses as Biomass for Industrial Manufacturing of Biofuel  Safety Hazards with Marine Products and Their Control: Food Borne Hazards, Algal Toxins, Control of Hazards.  Lab Work:  Field Trip: Survey of Phytoplankton and Zooplankton  Survey of marine vertebrates, Invertebrates,  Anatomy of farm fish (dissection of carp/trout/rohu, Isolation and characterization of microorganisms from fish, Marine and freshwater fishes of Pakistan (preserved specimens)

5.  DNA Extraction from Environmental Samples  Isolation, Culture, Staining & Biochemical Characterization of Marine Bacteria.  DNA Extraction from Marine Bacterial Culture  Algae Enumeration by MPN Method  Recommended Books:  Theory:  Marine Products for Healthcare: Functional and Bioactive Nutraceutical Compounds from the Ocean by Venugopal, V. Taylor & Francis Group.  Marine Biotechnology I by E Gal, Y., and Ulber, Springer.  Marine Biotechnology II by LE Gal, Y., and Ulber, Springer.  Microalgae as a Feedstock for Biofuels by Gouveia, L. Springer.  Marine Biology, 7 th Edition by Castro, P. and Huber, M.E. McGraw Hill Higher Education, U.S.A  Laboratory:  Environmental Microbiology: A Laboratory Manual. 2 nd Edition by Pepper, I.L., Gerba, C.P. 2004. Elsevier Academic Press.  Biotechnology Procedures & Experiments Handbook by Harisha, S. Infinity Science