1. September 18, 2012 Check the clipboard for your new seat I NTEGRATED S CIENCE D AY 6

2. L EARNING TARGETS 1. I can evaluate a laboratory procedure and analyze the data to form a conclusion

3. A GENDA Kick off question: How could you improve your laboratory skills? (do you need to follow directions better, work on being more precise, write a better conclusion, etc.) 1. Conclusion writing revisited 2. Daphnia Lab continued 3. Daphnia Lab conclusion 4. Turn in formal conclusion for heart rate lab 5. Turn in daphnia lab 6. Learning log: What does alcohol, caffeine and nicotine do to daphnia heartrate?

4. H OW TO WRITE A C ONCLUSION : You asked a question, you did an experiment, and you did the experiment a second time. You recorded your results. Now it is time to write your conclusion. The conclusion, plain and simple, is the answer to your question. It should be clear, concise and stick to the point. Resist the temptation to jump to conclusions. If you were to do your experiment again, would you get the same results? Can there be differences? Why? Ask yourself what happened when you tested your hypothesis. What have you learned from this experiment? What does this experiment tell us Now for homework-- using the answers to the questions on the heart rate lab, write a final report summarizing your question, research methods and conclusion. Work on your conclusion on a separate piece of paper until lunch. If you complete the conclusion, staple it to your lab report and turn it into the second period box.

5. R EVIEW OF M ICROSCOPE Use your assigned microscope and return it to its proper place as instructed Always start on low power Make sure your lenses are clean We will be using depression lenses, make sure your Daphnia is in the well and do not overflow.

6. B IOASSAYS Dose/Response Bioassays are used to: Estimate toxicity to humans. Estimate toxicity to humans. Estimate maximum concentrations of specific chemicals allowed to discharge into bodies of water. Estimate maximum concentrations of specific chemicals allowed to discharge into bodies of water. Investigate hazardous waste sites. Investigate hazardous waste sites.

7. 7 B IOASSAY S PECIES 3 organisms are often used Daphnia Daphnia Duckweed Duckweed Lettuce Seeds Lettuce Seeds Expose organisms to chemicals and measure how they respond.

8. R EMEMBER YOU ARE WORKING WITH A LIVING CREATURE http://www.microscopyu.com/moviegallery/pondsc um/daphnia/ At the end of each experiment return your daphnia to the Rehabilitation and Recovery area on the demonstration desk. Use a new daphnia for each chemical

9. D APHNIA

10. P ART 1 Take a look at your daphnia on low power-use the sketch I put on the overhead to help sketch and label your daphnia Look at your daphnia on medium power and sketch and label what you see Do not use high power with daphia or well plates

11. P ART 2 Make sure to get a new daphnia for each chemical you try You will need a timer to do the heart rate Make sure to record beats/min Return daphnia to the rehab bowl Clean up your lab station and return your microscope to the cabinet as directed

12. D ATA Make sure to put your averages into the data table at my desk Make sure to write down the class average Graph your data using a bar chart, showing your averages and the class averages

13. C ONCLUSION If time permits fill in the conclusion parts of the lab. Next class we will use this information and some background information about the drugs to build a more complete conclusion Turn in your lab report whether you complete the report or not.