1. SPECTROPHOTOMETER ANALYSIS Damian Ulrich and Sierra Werley

2. WHAT IS A SPECTROPHOTOMETER? An instrument that, when combined with a colorimeter in the lab, measures the amount of absorbance and transmittance a solution allows. The colorimeter is the device that allows the measurements to occur. Colorimeter Spectrophotometer

3. WHAT IS ABSORBANCE? A measure of the capacity of a substance to absorb light of a specified wavelength. Different colors of light rays have different wavelengths.

4. WHAT IS TRANSMITTANCE? The ratio of the light energy falling on a body to that transmitted through it.

5. WHAT TO DO You will be given concentration Use the spectrophotometer to find absorbance or transmittance Use a formula to find transmittance or absorbance (respectively) The formula is: A=2-Log(%T) A= absorbance %T= percent transmittance (T*100)

6. EXAMPLE Given: Concentration of CuSO 4 is.12M Spectrophotometer shows the %T is 23.2 Find the absorbance of the CuSO 4 in this situation. A= 2-log(%T) A= 2-log(23.2) A= 0.635 Au

7. QUIZ: QUESTION 1 Using a spectrophotometer, you find that the transmittance of a solution is 9.9% at a concentration of 0.20M. Find the absorbance of the solution. A. 0.98 Au B. 1.01 Au C. 0.50 Au D. 0.28 Au

8. QUIZ: QUESTION 2 Using a spectrophotometer, you find the absorbance to be 1.38Au of a solution with a concentration of 0.28M. Calculate the percent transmittance of the solution. A. 4.8 B. 3.6 C. 4.1 D. 10.3

9. QUIZ: QUESTION 3 Using a spectrophotometer, you find the absorbance to be 1.73Au of a solution with a concentration of 0.36M. Calculate the percent transmittance of the solution. A. 1.9 B. 0.23 C. 5.7 D. 3.2

10. QUIZ: QUESTION 4 Using a spectrophotometer, you find that the transmittance of a solution is 1.2% at a concentration of 0.40M. Find the absorbance of the solution. A. 1.00 Au B. 1.93 Au C. 2.57 Au D. 16.23 Au

11. QUIZ: QUESTION 5 You find the percent transition of an unknown solution to be 55.5%. Use the equation to find the absorbance of the same solution. A. 5.67 Au B. 1.34 Au C. 0.016 Au D. 0.259 Au

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23. WORKS CITED Thanks to Mr. Wolfe’s notes, the “Beer’s Law Lab” that came from Investigating Chemistry through Inquiry, and Google for definitions, this tutorial was able to be created.