1. Genomic stress influence on wing size and shape in Drosophila subobscura Author: Dijana Cvijetić Mentor: Aleksandra Patenković Institute for Biological Research “Siniša Stanković” Regional Center for Talented Youth Belgrade II, Serbia

2. Why Drosophila subobscura? Ecologically and genetically well characterized Easy and cheap to raise Rapid life cycle Large offspring Wing veins

3. Further explanations… Evolutionary biology Conservative genetics Other usage of geometric morphometrics

4. Inbreeding as genomic stress Inbreeding, mating between close relatives, is a genomic stress that may disrupt the stability of the genome. This phenomenon occurs in nature, especially in situations of habitat fragmentation.

5. The aim of the study was to analyze the direction and range of changes in wing size and shape of highly inbred lines of Drosophila subobscura. Objective

6. Materials and Methods Sample contained two genetically different populations from Belgrade Botanical Garden and Sicevo Gorge.

7. Thirteen generations were in full-sib inbreeding regime, and fourteenth generation was used. Also, heterozygote crosses were made. Flies were maintained on a standard cornmeal medium under optimal laboratory conditions.

8. Wings were landmarked with tpsDig 1.40 (13 landmarks). Wing size was examined using centroid size (CS) and shape was examined using weight matrix (WM). Procrustean superimposition calculation of the CS and WM was performed using tpsRelw 1.44.

9. Differences in wing size were measured with ANOVA (Analysis of Variance) and the ones in wing shape were measured with MANOVA (Multivariate Analysis of Variance).

10. Analyzed groups : SI - inbred lines from Sicevo Gorge BBI - inbred lines from Botanical Garden SBB - heterozygote cross between female from Sicevo Gorge and male from Botanical Garden BBS - heterozygote cross between female from Botanical Garden and male from Sicevo Gorge

11. Results In this table, basic results for CS were showed, where you can see number of analyzed wings (N), Mean value of CS and standard error of CS.

12. ANOVA This is a two way ANOVA table where Effect, Mean square of variances and F static with P value are shown.

13. Post hoc analysis Fishers LSD (least significant difference) post hoc test. Here are shown results for post hoc analysis between groups taking into account only one factor. (p<0.05 *, p<0.01 **, p<0.001***)

14. These are results from Fishers post hoc LSD test for two factors, group and sex. (p<0.05 *, p<0.01 **, p<0.001***)

15. Graphically presented differences in centroid size between each group and each sex.

16. MANOVA showed no difference in wing shape between experimental groups.

17. Conclusion It has been noted that the groups significantly differ among themselves, except homozygote groups, which indicates that inbreeding will have the same effect regardless of the origin of the tested population.

18. The end Thank you for your attention !