2. Summary: Context… Monitoring Goals… Methods… Results and Comparisons 2010 vs 2003 - Fish Size, DDT Skin-on vs Skin-off preparation method Other Contaminants Conclusions

5. Washington State Toxics Monitoring Program Goals Exploratory monitoring: since 2001. Characterize toxic contaminants at sites that lack data. Long Term monitoring: begin in 2009. Track contaminant levels over time at selected sites. Cooperative/complementary efforts; address new concerns.

9. Goals of WSTMP 2010 sample effort   Focus on Long Term Monitoring component   Lake Chelan, Wenatchee River, Banks Lake   Compare results: Historical vs 2010   Compare prep method: skin-on vs skin-off   Improve characterization of toxics in tissue   Support DOH data needs for review of FCA   Involve others: local gov’t, citizens, guides

12. Lake Chelan: 2010 effort   Mimic 2003 effort to collect/analyze lake trout   Time and location: 5/17/03 and 6/3/10 in Wapato Basin   Collection: H/L (derby in 2003, local guides in 2010)   Fish size: match weight range used in 2003 composites   Preparation: skin-on fillets both years (2010 add skin-off)   Lab Analyses DDT: EPA 8081 at MEL in 2003 and 2010   Lab Used: MEL; EPA-2003; ECY-2010

29. Results   DDT levels: 2010 and 2003   Compare skin-on vs skin-off preparation   Levels of other contaminants   Fish Consumption Advice

32. Boxplots of the 2003 and 2010 results for t-DDT

33. Summary of Two-Sample t-Tests for t-DDT in Samples from 2003 and 2010.

34.   Total DDT levels in lake trout from 2010 remain high.   DDT levels are higher than those in 2003.

38. Summary of Paired-Sample t-Test for Skin-On and Skin-Off Samples.

39.   Different fish preparation methods (skin-on vs skin-off) showed little or no difference in tissue DDT levels.

44. Conclusions   Total DDT levels in lake trout from 2010 remain high. Levels are higher than they were in 2003.   Different fish preparation methods (skin-on vs skin-off) showed little or no difference in tissue DDT levels.   Lake trout also have high levels of PBDE flame retardants, dioxins/furans, and elevated levels of PCBs.   Lake trout in Lake Chelan tend to be good biomagnifiers of environmental pollutants because they are: large, long-lived, fatty, and are at top of food web.

45. Contacts and Websites   Ecology - Fish Tissue Monitoring: Keith Seiders, 360-407-6689, Keith.Seiders@ecy.wa.gov.Keith.Seiders@ecy.wa.gov   Health – Fish Consumption Advice: Dave McBride, 360-236-3176, Dave.Mcbride@doh.wa.govDave.Mcbride@doh.wa.gov   Ecology Web site Toxic Hazards: http://www.ecy.wa.gov/toxhaz.html http://www.ecy.wa.gov/toxhaz.html   Health Web site - Environmental Health: http://www.doh.wa.gov/ehp/oehas/fish/ http://www.doh.wa.gov/ehp/oehas/fish/

46. Lab Method Differences between 2003 and 2010   Lipids: different sample prep (extraction solvents). Results not comparable.   Dioxins/furans: similar yet different methods. Results comparable for this study.   Mercury: different sample prep (rigor of digestion). Comparability of results good for most purposes when conversion factor used for estimations.