1. LAB 10 Physiology of the Circulatory system http://slowmuse.files.wordpress.com/2009/02/daphnia-15551.jpg http://library.thinkquest.org/05aug/01883/introtocircman.gif

2. 10A & B- Using a SPHYGMOMANOMETER SYSTOLE- Ventricles contract Artery pressure ↑ DIASTOLIC- Relaxation phase Artery pressure ↓

3. Blood pressure 120/70 – Average teenager Top number = systolic pressure Bottom number = diastolic pressure

4. 10 - C http://slowmuse.files.wordpress.com/2009/02/daphnia-15551.jpg

5. Set up Replace white stage disc with transparent glass disc Light from underneath http://www.bhphotovideo.com/c/product/497709-REG/Celestron_44202_Model_44202_Advanced_Stereoscopic.html

6. Effect of temperature on Daphnia heart rate Place rubberbands around microscope slide Place cotton ball in center Add 4-5 daphnia to cotton

7. Make a Daphnia “sandwich” Add 2 nd microscope slide on top of cotton with daphnia Secure with rubberbands

8. Fill petri dish with test water Place “daphnia sandwich” in water Place dish under microscope Count heart rate for 10 seconds X 6 Daphnia video