1. Phospho-mimic variant (T94E) of Tctex-1 Inventor: Ching-Hwa Sung, PhD Departments of Ophthalmology and Cell Biology Weill Cornell Medical College

2. Invention: Composition: T94E, gene and protein Uses: –Protein or gene therapy for CNS trauma and retinal nerve damage

3. Tctex-1 Known cargo-binding member of dynein complex Was puzzlingly found elsewhere in cell Phosphorylation status determines role –Unphosphorylated (T94A) >> dynein –Phosphorylated (T94E) >> other roles

4. Causes axonal and dendrite sprouting and growth (2005)

5. Partners with G proteins: role in maturation Tctex-1 = activator of G protein signaling 2 (AGS2)

6. Partners with G proteins

8. Renewal of stem cells

9. Other roles Causes axonal and dendrite sprouting and growth (2005) Gβγ is partner in this activity in development {Tctex-1 = activator of G protein signaling 2 (AGS2)} (2007) Phospho-Tctex-1 drives disasembly of primary cilium in neuronal progenitors >> self-renewing division Phosphorylation status determines role –Unphosphorylated (T94A) >> dynein/different. –Phosphorylated (T94E) >> sprouting/division

10. Developmental status –POC established in cells –Have many reagents: cDNAs, pAbs, mAbs, tg mouse Intellectual Property –2 US patents (7,439,328 on Composition; 8,168,749 method to use)7,439,3288,168,749 Publications –Chuang JZ et al (2005) The dynein light chain Tctex-1 has a dynein- independent role in actin remodeling during neurite outgrowth. Dev Cell. 9(1):75-86.Chuang JZ et al (2005) The dynein light chain Tctex-1 has a dynein- independent role in actin remodeling during neurite outgrowth. Dev Cell. 9(1):75-86. –Dedesma C, et al (2006) Dynein light chain Tctex-1 identifies neural progenitors in adult brain. J Comp Neurol. 496(6):773-86.Dedesma C, et al (2006) Dynein light chain Tctex-1 identifies neural progenitors in adult brain. J Comp Neurol. 496(6):773-86. –Sachdev P, et al (2007) G protein beta gamma subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth. EMBO J. 26(11):2621-32.Sachdev P, et al (2007) G protein beta gamma subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth. EMBO J. 26(11):2621-32.

11. Contact: Bruce Toman, CLP Technology Commercialization and Liaison Officer CCTEC 418 E 71st Street, Suite 61 New York, NY 10021 ph: 212-746-6187 toman@cornell.edu