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Copyright © 2009 Pearson Education, Inc. Art and Photos in PowerPoint ® Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter 10.

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Presentation on theme: "Copyright © 2009 Pearson Education, Inc. Art and Photos in PowerPoint ® Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter 10."— Presentation transcript:

1 Copyright © 2009 Pearson Education, Inc. Art and Photos in PowerPoint ® Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter 10 DNA Structure and Analysis Copyright © 2009 Pearson Education, Inc.

2 10.1The Genetic Material Must Exhibit Four Characteristics Replication Storage of information Expression of information Variation by mutation

3 Copyright © 2009 Pearson Education, Inc. Figure 10.1

4 Copyright © 2009 Pearson Education, Inc. 10.2Until 1944, Observations Favored Protein as the Genetic Material

5 Copyright © 2009 Pearson Education, Inc. Figure 10.2

6 Copyright © 2009 Pearson Education, Inc. 10.3Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages 10.3.1 Transformation: Early Studies

7 Copyright © 2009 Pearson Education, Inc. Figure 10.3

8 Copyright © 2009 Pearson Education, Inc. Table 10.1

9 Copyright © 2009 Pearson Education, Inc. 10.3Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages 10.3.2 Transformation: The Avery, MacLeod, and McCarty Experiment

10 Copyright © 2009 Pearson Education, Inc. Figure 10.4

11 Copyright © 2009 Pearson Education, Inc. 10.3Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages 10.3.3 The Hershey–Chase Experiment

12 Copyright © 2009 Pearson Education, Inc. Figure 10.5

13 Copyright © 2009 Pearson Education, Inc. Figure 10.6

14 Copyright © 2009 Pearson Education, Inc. 10.3Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages 10.3.4 Transfection Experiments

15 Copyright © 2009 Pearson Education, Inc. 10.4Indirect and Direct Evidence Supports the Concept that DNA Is the Genetic Material in Eukaryotes 10.4.1 Indirect Evidence: Distribution of DNA 10.4.2 Indirect Evidence: Mutagenesis

16 Copyright © 2009 Pearson Education, Inc. Table 10.2

17 Copyright © 2009 Pearson Education, Inc. Figure 10.7

18 Copyright © 2009 Pearson Education, Inc. 10.4Indirect and Direct Evidence Supports the Concept that DNA Is the Genetic Material in Eukaryotes 10.4.3 Direct Evidence: Recombinant DNA Studies

19 Copyright © 2009 Pearson Education, Inc. 10.5RNA Serves as the Genetic Material in Some Viruses

20 Copyright © 2009 Pearson Education, Inc. 10.6Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure 10.6.1 Nucleotides: Building Blocks of Nucleic Acids

21 Copyright © 2009 Pearson Education, Inc.

22 Figure 10.9

23 Copyright © 2009 Pearson Education, Inc. Figure 10.9a

24 Copyright © 2009 Pearson Education, Inc. Figure 10.9b

25 Copyright © 2009 Pearson Education, Inc. Figure 10.10

26 Copyright © 2009 Pearson Education, Inc. 10.6Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure 10.6.2 Nucleoside Diphosphates and Triphosphates

27 Copyright © 2009 Pearson Education, Inc. Figure 10.11

28 Copyright © 2009 Pearson Education, Inc. 10.6Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure 10.6.3 Polynucleotides

29 Copyright © 2009 Pearson Education, Inc. Figure 10.12

30 Copyright © 2009 Pearson Education, Inc. Figure 10.12a

31 Copyright © 2009 Pearson Education, Inc. Figure 10.12b

32 Copyright © 2009 Pearson Education, Inc. 10.7The Structure of DNA Holds the Key to Understanding Its Function 10.7.1 Base-Composition Studies 10.7.2 X-Ray Diffraction Analysis

33 Copyright © 2009 Pearson Education, Inc. Table 10.3

34 Copyright © 2009 Pearson Education, Inc. Figure 10.13

35 Copyright © 2009 Pearson Education, Inc.

36 10.7The Structure of DNA Holds the Key to Understanding Its Function 10.7.3 The Watson–Crick Model

37 Copyright © 2009 Pearson Education, Inc. Figure 10.14

38 Copyright © 2009 Pearson Education, Inc. Figure 10.14a

39 Copyright © 2009 Pearson Education, Inc. Figure 10.14b

40 Copyright © 2009 Pearson Education, Inc. Figure 10.14c

41 Copyright © 2009 Pearson Education, Inc. Figure 10.15

42 Copyright © 2009 Pearson Education, Inc. Figure 10.16

43 Copyright © 2009 Pearson Education, Inc. 10.8Alternative Forms of DNA Exist

44 Copyright © 2009 Pearson Education, Inc. Figure 10.17

45 Copyright © 2009 Pearson Education, Inc. Figure 10.17a

46 Copyright © 2009 Pearson Education, Inc. Figure 10.17b

47 Copyright © 2009 Pearson Education, Inc. 10.9The Structure of RNA Is Chemically Similar to DNA, but Single Stranded

48 Copyright © 2009 Pearson Education, Inc. Table 10.4

49 Copyright © 2009 Pearson Education, Inc. 10.10Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA 10.10.1Absorption of Ultraviolet Light (UV) 10.10.2Sedimentation Behavior

50 Copyright © 2009 Pearson Education, Inc. Figure 10.18

51 Copyright © 2009 Pearson Education, Inc. Figure 10.19

52 Copyright © 2009 Pearson Education, Inc. 10.10Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA 10.10.3Denaturation and Renaturation of Nucleic Acids

53 Copyright © 2009 Pearson Education, Inc. Figure 10.20

54 Copyright © 2009 Pearson Education, Inc. 10.10Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA 10.10.4Molecular Hybridization

55 Copyright © 2009 Pearson Education, Inc. Figure 10.21

56 Copyright © 2009 Pearson Education, Inc. 10.10Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA 10.10.5Fluorescent in situ Hybridization (FISH)

57 Copyright © 2009 Pearson Education, Inc. Figure 10.22

58 Copyright © 2009 Pearson Education, Inc. 10.10Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA 10.10.6Reassociation Kinetics and Repetitive DNA

59 Copyright © 2009 Pearson Education, Inc. Figure 10.23 C/C 0 = 1/(1+kC 0 t)

60 Copyright © 2009 Pearson Education, Inc. Figure 10.24

61 Copyright © 2009 Pearson Education, Inc. Figure 10.25

62 Copyright © 2009 Pearson Education, Inc. Figure 10.26

63 Copyright © 2009 Pearson Education, Inc. 10.10Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA 10.10.7Electrophoresis of Nucleic Acids

64 Copyright © 2009 Pearson Education, Inc. Figure 10.27


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