1. Copyright OpenHelix. No use or reproduction without express written consent1

2. FlyBase FlyBase: A resource for the genes, genome and molecular biology of Drosophila melanogaster and related species. Materials prepared by: Jennifer Williams, Ph.D. www.openhelix.com Updated: Q3 2010 Version 2

3. Copyright OpenHelix. No use or reproduction without express written consent3 FlyBase Agenda Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

4. Copyright OpenHelix. No use or reproduction without express written consent4 FlyBase Introduction http://www.flybase.org

5. Copyright OpenHelix. No use or reproduction without express written consent5 FlyBase Credits FlyBase is maintained by a consortium of Drosophila researchers and computer scientists located at: Harvard University University of Cambridge (UK) Indiana University.

6. Copyright OpenHelix. No use or reproduction without express written consent6 FlyBase Publication http://nar.oxfordjournals.org/cgi/reprint/37/suppl_1/D555

7. FlyBase User Guide Copyright OpenHelix. No use or reproduction without express written consent7

8. FlyBase Agenda Copyright OpenHelix. No use or reproduction without express written consent8 Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

9. Quick Searches Copyright OpenHelix. No use or reproduction without express written consent9 Click Help Select

10. Quick Searches Copyright OpenHelix. No use or reproduction without express written consent10 Click Fmr*

11. Quick Search Results Copyright OpenHelix. No use or reproduction without express written consent11 Click

12. Gene Report Page: Fmr1 Copyright OpenHelix. No use or reproduction without express written consent12

13. More Details Copyright OpenHelix. No use or reproduction without express written consent13 click

14. Polypeptide Data Section of Gene Report Copyright OpenHelix. No use or reproduction without express written consent14

15. Gene Ontology Section of Gene Report Copyright OpenHelix. No use or reproduction without express written consent15 Click Reference Report

16. FlyBase Agenda Copyright OpenHelix. No use or reproduction without express written consent16 Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

17. GBrowse Access Copyright OpenHelix. No use or reproduction without express written consent17 http://www.flybase.org Click

18. Copyright OpenHelix. No use or reproduction without express written consent18 Click Case-insensitive, allows wild cards GBrowse Homepage

19. GBrowse Overview & Details Panels Copyright OpenHelix. No use or reproduction without express written consent19 Gene mRNAs cDNAs Orthologs Alignments Transgene Mouse over for details Non coding RNA

20. GBrowse Tracks Copyright OpenHelix. No use or reproduction without express written consent20 Click here

21. Added Tracks to Details Panel Copyright OpenHelix. No use or reproduction without express written consent21 Added DNA/GC Removed CDS

22. Copyright OpenHelix. No use or reproduction without express written consent22 FlyBase Agenda Copyright OpenHelix. No use or reproduction without express written consent22 Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

23. Interactions Browser Access Copyright OpenHelix. No use or reproduction without express written consent23 http://www.flybase.org Select Click

24. Interactions Browser – Genetic Interactions Copyright OpenHelix. No use or reproduction without express written consent24 Click

25. Copyright OpenHelix. No use or reproduction without express written consent25 FlyBase Agenda Copyright OpenHelix. No use or reproduction without express written consent25 Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

26. Accessing QueryBuilder Copyright OpenHelix. No use or reproduction without express written consent26 Click http://www.flybase.org

27. QueryBuilder Search Example Copyright OpenHelix. No use or reproduction without express written consent27 Click 8

28. Copyright OpenHelix. No use or reproduction without express written consent28 Building Your Query Select

29. Copyright OpenHelix. No use or reproduction without express written consent29 Click Selecting Search Terms Browse CLICK

30. QueryBuilder Output Options & Submission Copyright OpenHelix. No use or reproduction without express written consent30 Click to remove CLICK Click to add segment Click to modify segment

31. QueryBuilder Results Copyright OpenHelix. No use or reproduction without express written consent31 Gene Report CLICK

32. Copyright OpenHelix. No use or reproduction without express written consent32 FlyBase Agenda Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

33. Accessing Sequence Searches (BLAST) Copyright OpenHelix. No use or reproduction without express written consent33 http://www.flybase.org Select

34. Building Your BLAST Query Copyright OpenHelix. No use or reproduction without express written consent34 Click Select

35. Inputting Your Sequence Copyright OpenHelix. No use or reproduction without express written consent35

36. More BLAST Options Copyright OpenHelix. No use or reproduction without express written consent36

37. Retrieving BLAST Results Copyright OpenHelix. No use or reproduction without express written consent37 Click BLAST Report

38. Sequence Searches (BLAST) Results Page Copyright OpenHelix. No use or reproduction without express written consent38

39. Copyright OpenHelix. No use or reproduction without express written consent39 FlyBase Agenda Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

40. A Wealth of Features 40Copyright OpenHelix. No use or reproduction without express written consent

41. Additional Tools: TermLink Copyright OpenHelix. No use or reproduction without express written consent41 Click Matching CV terms Nucleolus Homepage Access

42. TermLink Term Report Copyright OpenHelix. No use or reproduction without express written consent42

43. Additional Tools: ImageBrowse Copyright OpenHelix. No use or reproduction without express written consent43 Homepage Access Image Report CV Term Report

44. Additional Tools: CytoSearch Copyright OpenHelix. No use or reproduction without express written consent44 2F1-2F1 Sequence information via GBrowse Gene Report Page Stock Repor t

45. Additional Tools: Chromosome Maps Copyright OpenHelix. No use or reproduction without express written consent45 Corresponding GBrowse window

46. Additional Tools: Coordinate Converter Copyright OpenHelix. No use or reproduction without express written consent46 Old Coordinates New Coordinates Conversion Report

47. Copyright OpenHelix. No use or reproduction without express written consent47 FlyBase Agenda Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

48. Summary The most current information for Drosophilidae Content updated regularly Easily searchable topics - from molecular biology to classical genetics Freely available to the public and for download Copyright OpenHelix. No use or reproduction without express written consent48 Extensive Gene Reports Sequence searches Interactions

49. Copyright OpenHelix. No use or reproduction without express written consent49 FlyBase Agenda Introduction and Credits Quick Search and Gene Report Page GBrowse Interactions Browser QueryBuilder Sequence Searches (BLAST) Additional Tools/Features Summary Exercises FlyBase: http://www.flybase.orghttp://www.flybase.org

50. Copyright OpenHelix. No use or reproduction without express written consent50