1. [1] 3D Micromanufacturing Lab. CO 2 INCUBATOR

2. [2] 3D Micromanufacturing Lab. What is an Incubator In biology, an incubator is a device used to grow and maintain microbiological cultures or cell cultures. It maintains optimal temperature, humidity and other conditions such as the carbon dioxide (CO 2 ) and oxygen content of the atmosphere inside.temperature, humiditycarbon dioxideoxygen The most commonly used setting used for storing MCF-7 and MDAMB231 cells in an incubator are 37 °C and CO 2 level of 5%.

3. [3] 3D Micromanufacturing Lab. Components 1.Water tray. 2.Stainless steel alloy interior 3.Direct Heat and Air Jacket heating system 4.Thermal conductivity CO 2 sensor 5.Outer door heater 6.Door-mounted microprocessor 7.Adjustable levelling feet 8.Removable interior components 9.Rounded, electro-polished interior corners for easy cleaning

4. [4] 3D Micromanufacturing Lab. Settings Normal settings for storing MCF-7 and MDAMB231 cells. If temperature or CO 2 supply is below the set values, buzzer will beep. CO 2 supply pressure should be 0.5 bar. Autoclaved water should be filled in the water tray. CO 2 Supply Cylinder Incubator Panel

5. [5] 3D Micromanufacturing Lab. Specifications MCO-18AC-PA Maximum Power Consumption 310W Maximum Heat Discharge1116kJ/h Electrical Connection 115V, 60Hz, 1 phase, NEMA 5- 15P plug CO 2 Gas Connection0.25" barbed fitting CO 2 Gas Input Pressure 5 psi from two-stage CO 2 regulator CO 2 Gas Cylinder Switchover System Optional, MCO-21GC, internal