1. 海洋天然物 Elysia rufescens 海蛞蝓 Kahalalide F (KF) 指導老師：詹于誼 組員：楊仲智 張芳瑛 翁曼真

2. 0.2 to 4  M using a continuous 96 hr(median = 0.75  ).

3. Images of cells treated with KF 5  M for PLC/PRF/5C 0.5  M for HepG2 KFcontrol

4. 4–6  M KF in PLC/PRF/5C cell 5M5M 2.5  M 1M1M 0.5–0.7  M KF in HepG2 cells 0.1  M 1M1M 2.5  M 5M5M 0.5  M 10  M 25  M KF-induced depletion of ATP in HepG2 and PLC/PRF/5C cells

5. Analysis of KF-induced permeability changes in HepG2 cells by flow cytometry (a) control

6. (b) treated with 0.25  M KF for 1 h

8. LDH release by HepG2 and PLC/PRF/5C after exposure to KF for 1 h. HepG2 PLC/PRF/5C

9. Discussion HepG2 cells demonstrated profound ATP depletion, associated with cell swelling and cell blebbing, and increased permeability to propidium iodide (PI), annexin V (AV) and release of lactate dehydrogenase (LDH).cell membrane induced by KF in HepG2 PLC/PRF/5C cells retained their cell structure, but were permeable to PI and, following exposure to high concentrations of KF, to AV. PLC/PRF/5C suggest specific interactions with membranes or proteins. This could lead to better drug design aimed at exploiting the potential for cell selectivity.