1. Supp Fig. 1

2. Protein numberScores (>28=p<0.05)Name of peptide GINumber 11148myosin, heavy polypeptide 4, skeletal muscle67189167 2682myosin, heavy polypeptide 8, skeletal muscle, perinatal71143152 3627myosin, heavy polypeptide 1, skeletal muscle, adult82524274 4497myosin, heavy polypeptide 2, skeletal muscle, adult205830428 5448desmin33563250 6289alpha-cardiac actin387090 7240gamma-actin809561 8210Myh7 protein187956918 9197alpha cardiac myosin heavy chain191618 10197fast skeletal myosin alkali light chain 1 isoform 1f29789016 11188myosin, heavy polypeptide 13, skeletal muscle124486959 12169glyceraldehyde-3-phosphate dehydrogenase6679937 13168muscle embryonic myosin heavy chain 3153792649 14165 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 16680748 15161ATP synthase beta-subunit2623222 16159 PREDICTED: similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)149265922 17158muscle creatine kinase6671762 18154tropomyosin 2, beta11875203 19154spermatid-specific556310 20151alpha-tropomyosin157787199 21139ATPase, Ca++ transporting, fast twitch 136031132 22123filamin C, gamma124487139 23113heat shock protein HSP27424143 2492parvalbumin53819 2590 PREDICTED: similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)149265502 2684H3 histone, family 3A4504279 2782albumin26340966 2880fructose-bisphosphate aldolase A6671539 2978TI-2251167510 3069mCG13235148684097 3168elongation factor 1alpha50797 3266enolase 3, beta muscle6679651 3365elongation factor 226328763 3463Histone H2A51304 3561PDZ-LIM protein cypher1c (ZASP)11612596 3658PDZ and LIM domain protein 37948997 3755M2-type pyruvate kinase1405933 3852RecName: Full=Titin; AltName: Full=Connectin160358754 3950sarcalumenin26331358 4049 Chain A, Crystal Structure Of Macrophage Migration Inhibitory Factor Complexed With (E)-2-Fluoro-P-Hydroxycinnamate5542285 4148myosin light chain, phosphorylatable, fast skeletal muscle7949078 4248tubulin, beta, 25174735 4348tubulin, beta 627754056 4448tubulin, beta 24507729 4548tubulin, beta 312963615 4648fast skeletal muscle troponin C6678371 4745tubulin beta-1 chain - mouse91855 4843heat shock protein 70 cognate309319 4943 RecName: Full=Hemoglobin subunit alpha; AltName: Full=Hemoglobin alpha chain; AltName: Full=Alpha-globin122441 504116S ribosomal protein200796 5139pro-alpha-2(I) collagen50489 5239phospholipase c74180086 5333cytochrome c oxidase subunit Va preprotein50527 5430Hspa8 protein63101351 5529reductase superfamily containing protein26329759 5628malate dehydrogenase387422 Supplementary Material List 1: Proteins identified in purified aggregates

3. Proteinscore (>27=p<0.05)Name of peptideGI Number 11538myosin, heavy polypeptide 4, skeletal muscle67189167 21309myosin, heavy polypeptide 1, skeletal muscle, adult82524274 3939myosin, heavy polypeptide 2, skeletal muscle, adult56206250 4924 Myosin, heavy polypeptide 8, skeletal muscle, perinatal187956525 5850 myosin, heavy polypeptide 8, skeletal muscle, perinatal71143152 6340tropomyosin 2, beta11875203 7335myosin, heavy polypeptide 7, cardiac muscle, beta18859641 8314alpha-cardiac actin387090 9292alpha-tropomyosin157787199 10273myosin, heavy polypeptide 13, skeletal muscle124486959 11233actin, beta, cytoplasmic74178253 12228muscle creatine kinase 6671762 13225mCG140437, isoform CRA_a148678478 14213fructose-bisphosphate aldolase A6671539 15211fast skeletal myosin alkali light chain 1 isoform 1f29789016 16119 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 16680748 17107ATPase, Ca++ transporting, fast twitch 136031132 1888adenine nucleotide translocase-1902008 1986glyceraldehyde-3-phosphate dehydrogenase6679937 2083parvalbumin53819 2182desmin148667985 2271Atp5b protein23272966 2360actinin alpha 37304855 2457enolase 3, beta muscle6679651 2556phosphoglycerate mutase 29256624 2656ATP synthase beta-subunit2623222 2753M-protein2950347 2850 PREDICTED: similar to Glyceraldehyde-3- phosphate dehydrogenase (GAPDH)149265502 2950 myosin light chain, phosphorylatable, fast skeletal muscle7949078 3049 ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e 26373885 3147fast skeletal muscle troponin C 6678371 3244myoglobin21359820 3342 RecName: Full=Hemoglobin subunit alpha; AltName: Full=Hemoglobin alpha chain; AltName: Full=Alpha-globin 122441 3437spermatid-specific556310 3536troponin T2340050 36 RecName: Full=Neuronal cell adhesion molecule; Short=Nr-CAM; AltName: Full=NgCAM-related cell adhesion molecule; Short=Ng38372441 3733myozenin 110946924 3831TAFI951842208 3931transmembrane protein TM9SF1 9755055 4026alpha-fetoprotein191765 4126 PREDICTED: similar to Glyceraldehyde-3- phosphate dehydrogenase149260720 Supplementary Material List 2: Proteins identified in purified aggregates