1. Teaching Genetic Linkage and Recombination through Mapping with Molecular Markers Note for instructors: Please see the notes field for answers. 1 Lisa McDonnell & Jennifer Klenz Gels to analyze as part of the Post-Class Tutorial (or homework) problem set (see Supplemental Material S5a for the problem set)

2. Jeff x Pio  F1 TESTCROSS: F1 x Jeff tester  F2 Marker #1.1 #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 #14 #15 #16 #17 #18 #19 #20 #21 CONTROLS Jeff Pio F1 (parent)) (parent) F2 not resistant trees F2 disease resistant trees #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 #14 #15 #16 #17 #18 #19 #20 #21 #22 #23

3. #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 #14 #15 #16 #17 #18 #19 #20 #21 CONTROLS Jeff x Pio  F1 TESTCROSS: F1 x Jeff tester  F2 Marker #4.1 Jeff Pio F1 (parent)) (parent) F2 not resistant trees F2 disease resistant trees #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 #14 #15 #16 #17 #18 #19 #20 #21 #22 #23

4. Jeff x Pio  F1 F1 self-fertilized  F2 Marker #1.1 #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 #14 #15 #16 #17 #18 #19 #20 #21 Disease resistant F2s only Jeff Pio F1 (parent)) (parent) CONTROLS Not resistant F2s only #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 #14 #15 #16 #17 #18 #19 #20 #21 #22 #23

5. #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 #14 #15 #16 #17 #18 #19 #20 #21 CONTROLS Jeff x Pio  F1 F1 self-fertilized  F2 Marker #4.1 Jeff Pio F1 (parent)) (parent) F2 not resistant trees F2 disease resistant trees #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 #13 #14 #15 #16 #17 #18 #19 #20 #21 #22 #23