Presentation is loading. Please wait.

Presentation is loading. Please wait.

Harmful algal bloom (HAB)-on-a-chip: Development of a microfluidic platform to study algal chemotaxis Kelly O’Quinn Dr. Adam Melvin Cain Department of.

Published byDale Watts Modified over 9 years ago

Similar presentations

Presentation on theme: "Harmful algal bloom (HAB)-on-a-chip: Development of a microfluidic platform to study algal chemotaxis Kelly O’Quinn Dr. Adam Melvin Cain Department of."— Presentation transcript:

1 Harmful algal bloom (HAB)-on-a-chip: Development of a microfluidic platform to study algal chemotaxis Kelly O’Quinn Dr. Adam Melvin Cain Department of Chemical Engineering Louisiana State University

2 Harmful Algal Blooms Overabundance of algae Problems Reproduction and
Karenia brevis Problems Ecosystem Economy Health Reproduction and Migration

3 Migration of Algae Phototaxis: upward migration toward light during day Chemotaxis: downward migration toward nutrients at night

4 Heterogeneous Environments
Algae migrate in response to many factors Concentrations not constant throughout system

5 Developing a New Technology
Microfluidic devices Precise control of fluids Laminar flow: diffusion-based mixing Dynamic control Past research Homogeneous tanks Other microfluidic devices

6 Design and Fabricate the Device
Design in AutoCAD Soft lithography Master PDMS replication

7 Fabrication Spin coat photoresist onto wafer
Place photomask onto wafer then UV expose then bake Cross-linked polymers stay and non cross- linked dissolve in solution

8 Device Assembly Layers Tubes Options for adhering layers PDMS Agarose
Glass slide Tubes Options for adhering layers Silanes Thiol-ene resin Plexiglas box Negative pressure Shing-Yi Cheng, Steven Heilman, Max Wasserman, Shivaun Archer, Michael L. Shuler and Mingming Wu, “A hydrogel-based microfluidic device for the studies of directed cell migration,” Lab Chip, 2007, 7,

9 Characterize the Device
Ensure stable, well-controlled gradients formed Fluorescent tracers ImageJ line scan COMSOL models

10 Implement the Device for Studying Algae Migration
Chlamydomonas reinhardtii Unicellular, survives in many environments Load both channels Allow gradient to develop Load cells into center, flow-free channel

11 Gathering and Analyzing Results
Chemotactic Index Vector assigning Design of Experiment (DOE) Analysis of Variance (ANOVA)

12 Future Applications Better understanding of algae migration
Most prominent factors causing blooms Developing new technology Future collaborations with other labs

Download ppt "Harmful algal bloom (HAB)-on-a-chip: Development of a microfluidic platform to study algal chemotaxis Kelly O’Quinn Dr. Adam Melvin Cain Department of."

Similar presentations

FABRICATION PROCESSES

FABRICATION PROCESSES
Process Flow : Overhead and Cross Section Views ( Diagrams courtesy of Mr. Bryant Colwill ) Grey=Si, Blue=Silicon Dioxide, Red=Photoresist, Purple= Phosphorus.

Process Flow : Overhead and Cross Section Views ( Diagrams courtesy of Mr. Bryant Colwill ) Grey=Si, Blue=Silicon Dioxide, Red=Photoresist, Purple= Phosphorus.
Device Design and Fabrication Using lithography techniques, a Y-channel master was fabricated with SU8 photoresist. Master on Silicon With this master,

Device Design and Fabrication Using lithography techniques, a Y-channel master was fabricated with SU8 photoresist. Master on Silicon With this master,
Metamaterials Zaven Kalfayan Lindsay Hunting Phyllis Xu Joy Perkinson.

Metamaterials Zaven Kalfayan Lindsay Hunting Phyllis Xu Joy Perkinson.
Photolithography PEOPLE Program July 8, 2013. Computer chips are made using photolithography Instead of drawing with a sharp tip, it uses light to transfer.

Photolithography PEOPLE Program July 8, Computer chips are made using photolithography Instead of drawing with a sharp tip, it uses light to transfer.
Prototype Showcase  What is a metamaterial?  How our 2-D sample was created  How our phase mask was created  SEM images of 2-D sample and phase mask.

Prototype Showcase  What is a metamaterial?  How our 2-D sample was created  How our phase mask was created  SEM images of 2-D sample and phase mask.
Microfluidic Valve Innovation Jo Falls Porter, RET Fellow 2009 West Aurora High School RET Mentor: Dr. David T. Eddington, PhD NSF- RET Program Introduction.

Microfluidic Valve Innovation Jo Falls Porter, RET Fellow 2009 West Aurora High School RET Mentor: Dr. David T. Eddington, PhD NSF- RET Program Introduction.
MONOLITHIC 3-D MICROFLUIDIC DEVICE FOR CELL ASSAY WITH AN INTEGRATED COMBINATORIAL MIXER 陳睿鈞 Mike C. Liu, Dean Ho, Yu-Chong Tai Department of Bioengineering,

MONOLITHIC 3-D MICROFLUIDIC DEVICE FOR CELL ASSAY WITH AN INTEGRATED COMBINATORIAL MIXER 陳睿鈞 Mike C. Liu, Dean Ho, Yu-Chong Tai Department of Bioengineering,
CISMM: Computer Integrated Systems for Microscopy and Manipulation Collaborators: Dr. R. E. Cheney, Dept of Cell and Molecular Physiology Project Lead:

CISMM: Computer Integrated Systems for Microscopy and Manipulation Collaborators: Dr. R. E. Cheney, Dept of Cell and Molecular Physiology Project Lead:
Optimal Conditions for Cell Viability on SU-8 Adherent Cell Sorting Microstructures Julius Oatts Emory University – Neuroscience and Behavioral Biology.

Optimal Conditions for Cell Viability on SU-8 Adherent Cell Sorting Microstructures Julius Oatts Emory University – Neuroscience and Behavioral Biology.
Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography EECE 491C Unger et al. (Quake group, CALTECH) Science, 2000.

Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography EECE 491C Unger et al. (Quake group, CALTECH) Science, 2000.
Fabrication of Microfluidic Devices for 3D Chemotaxis Studies Delaram Sahebzamani University of South Florida Mentor: Noo Li Jeon Department of Biomedical.

Fabrication of Microfluidic Devices for 3D Chemotaxis Studies Delaram Sahebzamani University of South Florida Mentor: Noo Li Jeon Department of Biomedical.
Microbubble Generation for Medical Applications Shirley Zhang Faculty Mentor: Abraham Lee Graduate Student: Kanaka Hettiarachchi.

Microbubble Generation for Medical Applications Shirley Zhang Faculty Mentor: Abraham Lee Graduate Student: Kanaka Hettiarachchi.
A PDMS DIFFUSION PUMP FOR ON-CHIP FLUID HANDLING IN MICROFLUIDIC DEVICES Mark A. Eddings and Bruce K. Gale Department of Bioengineering, University of.

A PDMS DIFFUSION PUMP FOR ON-CHIP FLUID HANDLING IN MICROFLUIDIC DEVICES Mark A. Eddings and Bruce K. Gale Department of Bioengineering, University of.
Zarelab Guide to Microfluidic Lithography Author: Eric Hall, 02/03/09.

Zarelab Guide to Microfluidic Lithography Author: Eric Hall, 02/03/09.
Basic Silicone Chemistry (II)

Basic Silicone Chemistry (II)
Lecture 10.0 Photoresists/Coating/Lithography. Semiconductor Fab Land$0.05 Billion Building$0.15 Billion Tools & Equipment $1 Billion Air/Gas Handling.

Lecture 10.0 Photoresists/Coating/Lithography. Semiconductor Fab Land$0.05 Billion Building$0.15 Billion Tools & Equipment $1 Billion Air/Gas Handling.
NANOSCALE LITHOGRAPHY MICHAEL JOHNSTON 4/13/2015.

NANOSCALE LITHOGRAPHY MICHAEL JOHNSTON 4/13/2015.
Lithographic Processes

Lithographic Processes
Microfluidics: Catalytic Pumping Systems Paul Longwell Hollidaysburg Area High School Summer 2005.

Microfluidics: Catalytic Pumping Systems Paul Longwell Hollidaysburg Area High School Summer 2005.

Similar presentations

Ads by Google