1. Eukaryotic Translation mRNA tRNA rRNA

4. -monocistronic -1,000-2,000 bases long -methylated at 5’cap -100-200 bases of poly A at 3’end -nontranslated 5’ reader < 100 bases -nontranslated 3’ tailer ~ 1,000 bases - AUG as the INITIATOR -UAA,UAG,&UGA as TERMINATOR mRNA

6. Fig5.16 Expression of mRNA

7. -removal of Poly(A) need for decapping enzyme -Poly(A)-binding protein, PABP on Poly(A) prevents the decapping enzyme from binding to the 5’end Life Cycle of Eukaryotic mRNA

8. AU-rich sequence, called ARE ~50 bases in 3’ tailer -AUUUA, more repeated -trigger destabilization

9. TRE sequence - contain strm-loop structures for protein binding -Protein inhibit destabilizing

10. tRNA Met-tRNA i for initiation Met-tRNA m for elongation - No formylated Methionine -has an unusual tertiary structure, modofied by phosphorylation of the 2’ribose position

11. Eukaryotic Ribosome

12. The right context for Recognization -AUG -the most important seq. GCC A G CCAUGG, purine three bases before AUG and G immediately following it. CAP; for a stable complex to be formed at initiation codon

13. Ternary complex; -Met-tRNA i -eIF2 -GTP Eukaryotic Initiation *form ternary complex *bind free 40S *attach the 5’ end

14. Initiation Factors *bind 5’ cap *unwind mRNA *bind subunit initiation complex *support 60S joining

15. Elongation Factors: EF-Tu, load aminoacyl tRNA EF-Ts, GDP to GTP

16. Translocation: Peptidyl transferase, synthesis peptide

17. EF-Tu EF-G

19. Fig.6.27