1. lP2HRI Wellesbourne lP3University of Liverpool WP4Garlic Sulphur Biochemistry lTo establish the limiting biochemical and physiological steps for allicin formation in garlic lBiochemical pathway (Liverpool) lIntegration with development (HRI) lGenes for limiting steps (Liverpool / HRI) Partners responsible Objectives

2. GARLIC & HEALTH Pathway Biochemistry

3. GARLIC & HEALTH Pathway Biochemistry Biochemistry CSO 3 Alliinase PyruvateAmmonia S alk(en)yl sulfenic acids Allicin Organosulphurcompounds Tissue damage

4. GARLIC & HEALTH Integration with development

5. ãcDNA libraries ãPathway intermediates ãSulphur budget ãGenes (promoters) for key enzymes ãPublications ãTargets for breeding, MAS identified GARLIC & HEALTH Deliverables

6. DELIVERABLES 4DP. 8: Analytical methods for labeling and analysis (P2, P3) 4DP. 9: A cDNA library from garlic (P2) 4DP. 16: Pathway intermediates identified (P3) 4DP. 17: First sulphur budget for garlic (P2) 4DP. 18: Clones for alliinase (P2) lDP. 23: Publication on alliin biosynthesis and sulphur partitioning (P2, P3) 4DP. 24: Genes for key CSO synthesis enzymes (P2,P3) mDP. 29: Publication on the characterisation of key enzymes in alliin biosynthesis (P2, P3, P5) lDP. 35: Publication on the regulation of alliinase expression (P2, P3) lDP. 36: Publication on the regulation of sulphur biochemistry in garlic (P2, P3, P5)  = Achieved = delayed  = future deliverable

7. YEAR 3 MILESTONES 4Analysis of pattern of labeling in later stage intermediates completed (P3). lAnalysis of second-year field experiment completed (P2). lWhole plant labeling studies completed (P2). mPublication on the developmental control of alliin synthesis submitted (P2). mPurification of PCR amplified cDNA and sequencing completed (P3). 4Expression studies on alliinase clones initiated (P2).  = Achieved = Subsequently completed  =To be completed