1. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme Heating Substrate Cooling Enzyme Cooling Substrate pH More acidic (lower pH) More basic (higher pH) Concentration Increasing enzyme conc Increasing substrate conc

2. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme STOPSEnzyme denatures in heat Heating Substrate Cooling Enzyme Cooling Substrate pH More acidic (lower pH) More basic (higher pH) Concentration Increasing enzyme conc Increasing substrate conc

3. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme STOPSEnzyme denatures in heat Heating Substrate DecreasesMore Molecular Kinetic Energy Cooling Enzyme Cooling Substrate pH More acidic (lower pH) More basic (higher pH) Concentration Increasing enzyme conc Increasing substrate conc

4. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme STOPSEnzyme denatures in heat Heating Substrate DecreasesMore Molecular Kinetic Energy Cooling Enzyme Increases Less Molecular Kinetic Energy Cooling Substrate pH More acidic (lower pH) More basic (higher pH) Concentration Increasing enzyme conc Increasing substrate conc

5. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme STOPSEnzyme denatures in heat Heating Substrate DecreasesMore Molecular Kinetic Energy Cooling Enzyme Increases Less Molecular Kinetic Energy Cooling Substrate Increases Less Molecular Kinetic Energy pH More acidic (lower pH) More basic (higher pH) Concentration Increasing enzyme conc Increasing substrate conc

6. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme STOPSEnzyme denatures in heat Heating Substrate DecreasesMore Molecular Kinetic Energy Cooling Enzyme Increases Less Molecular Kinetic Energy Cooling Substrate Increases Less Molecular Kinetic Energy pH More acidic (lower pH) Increases Enzymes have an optimal pH; outside the range cause it to denature More basic (higher pH) Concentration Increasing enzyme conc Increasing substrate conc

7. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme STOPSEnzyme denatures in heat Heating Substrate DecreasesMore Molecular Kinetic Energy Cooling Enzyme Increases Less Molecular Kinetic Energy Cooling Substrate Increases Less Molecular Kinetic Energy pH More acidic (lower pH) Increases Enzymes have an optimal pH; outside the range cause it to denature More basic (higher pH) Increases ” Concentration Increasing enzyme conc Increasing substrate conc

8. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme STOPSEnzyme denatures in heat Heating Substrate DecreasesMore Molecular Kinetic Energy Cooling Enzyme Increases Less Molecular Kinetic Energy Cooling Substrate Increases Less Molecular Kinetic Energy pH More acidic (lower pH) Increases Enzymes have an optimal pH; outside the range cause it to denature More basic (higher pH) Increases ” Concentration Increasing enzyme conc Decreases Assuming there is enough substrate; more enzyme increase the number of collisions and speeds up the reaction Increasing substrate conc

9. VariableReaction Time…Why does this Happen? Temperature Heating Enzyme STOPSEnzyme denatures in heat Heating Substrate DecreasesMore Molecular Kinetic Energy Cooling Enzyme Increases Less Molecular Kinetic Energy Cooling Substrate Increases Less Molecular Kinetic Energy pH More acidic (lower pH) Increases Enzymes have an optimal pH; outside the range cause it to denature More basic (higher pH) Increases ” Concentration Increasing enzyme conc Decreases Assuming there is enough substrate; more enzyme increase the number of collisions and speeds up the reaction Increasing substrate conc Decreases More substrate (H 2 O 2 ) increases the number of collisions until the point in which the enzyme is saturated

10. What to study for Biochemistry Test?? Chapter 4 – Atom structure, bonds, properties of water, pH scale, energy of reactions Chapter 5 – Compare and Contrast macromolecules – How to build a polymer and break it apart – Enzyme structure, function, and reactions Use study guide, labs, practices, homework, and read the chapters!!