1. BIOE/CHBE 460 Biochemical Engineering

2. Early penicillin culture facility at the Sir William Dunn School of Pathology, Oxford, England. © Museum of the History of Science, Oxford Merck Archives, ©Merck & Co. Inc.

3. Example of process improvement

4. Major advancements Molecular biology Genetic engineering Cell and tissue culture

8. Examples of genetic engineering application (first generation application)

9. Recombinant proteins by microorganisms Restriction sites Ligation Transformation Cloning vector Recombined plasmid Restriction cleavage Restriction cleavage Gene of interest Transcription Translation mRNA Protein Host cell

10. Recombinant proteins by microorganisms YearProducts DiseaseCompany 1982Humulin Type 1 diabetesGenetech, Inc. (synthetic insulin) 1985ProtropinGrowth hormone Genetech, Inc. Deficiency Some early products

11. Examples of pathway and metabolic engineering (second generation application of genetic engineering)

12. Introduction What is pathway and metabolic engineering?

15. chilling tolerant plant*

16. Cell and tissue cultures monoclonal antibody by hybridoma cells

17. Monoclonal Antibody screen for desired clone only hybridoma cells grow cells grown in HAT medium hybridoma myeloma cells antigen injected into mouse spleen removed B lymphocytes

18. Applications of Monoclonal Antibodies Diagnostic ELISA Tests – PSA, AIDS, pregnancy Passive Immunization Detection, isolation and purification of biomolecules

20. by Genentech, Corporate Communication A Fermenter / Bioreactor And Its Parts

21. Separation

22. Chromatography Columns BioProcess Glass (BPG) Columns Amersham Pharmacia Biotech

23. Single System for Anchorage-Dependent and Suspension Cultures New Brunswick Scientific Company