1. Diffusion, Microscope & Cell Lab02

2. Activity 1 Diffusion experiment Methylene blue MW 320g/mole
Potassium permagnate MW 150 g/mole
Form Hypothesis
T0 initial diameter T60 final diameter
Room temp
Fridge

3. Carrying a Microscope A B

4. Storing The Microscope
Return the lowest power objective in place
Wrap the cord around the base
Return dustcover

5. Cleaning the Microscope
Use lens paper on all glass parts of the microscope.
Clean oil immersion lens with chemicals provided by your instructor

6. Parts of the Microscope
Ocular
Body tube
Nosepiece
Objective lenses (10, 40, 100x)
Arm
Course adjustment knob
Fine adjustment knob
Stage
Stage clips
Aperture
Diaphragm
Light
Base

7. Microscope Parts Ocular Body tube Stage clip Revolving nose piece
Objective
Arm
Stage
Diaphragm
Lever to move stage clip
Course adjustment
Fine adjustment
Light source
Base

8. Using the microscope
Always observe using the LOWEST POWER objective first.
Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Bring the object into sharp focus by using the fine adjustment knob.
Focus, and then move to a higher power objective, if needed.
Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE.
Keep both eyes open to reduce eyestrain.
Determine total magnification of the object by multiplying the power of the ocular (10x) the power by the power of the objective.

9. Preparing a slide
Using a pipet or dropper, add a drop of water or another solvent to a clean microscope slide. Then, place the specimen in the water.
Place the edge of a coverslip on the slide so that it touches the edge of the water.
Slowly lower the coverslip to prevent the formation of air bubbles.

10. Oil Immersion Lens
100x
40x

11. Animal Cell

12. Plant Cell

13. Activity 2 Prepare wet mount Cheek cell Elodea cell Onion cell
Look at specimen under high power and draw what you see.
Use proper clean-up technique

14. Cheek Cell

15. Elodea

16. Epidermal Onion Cell

17. Activity 3 Prepare wet mount for dissecting scope Pond water
Look at specimen under high power and draw what you see.
Use proper clean-up technique

18. Dispose Biological material

19. Dispose Sharps

20. Clean Up

21. Microscope Parts Quiz

22. Microscope Quiz Which objective uses oil?
If your ocular is 10x and your objective is 40x what is the total magnification of your image?
Why do you need a cover slip and how do you avoid an air bubble?
What is the difference between a light microscope and a dissecting microscope?
1.    

23. Instructor’s Notes: Materials & Equipment 5 dissecting scopes 5 Rulers
5 light microscopes
Cover slips
Slides (box)
Droppers
Water in dropper
Oil and cleaner for oil immersion lens
Lens paper
Onion
Elodea
Toothpicks (box)
Sharps and biohazard bag
disinfectant
5 Rulers
10 agar plates plus a few extra
Methylene blue
Potassium permagnate
Razor blade to cut plants
Pondwater
Use of fridge