1. Screening for Lactobacilli in Dairy Products

2. Probiotics in Dairy Products Definition: Live microorganisms which when administered in adequate amounts confer a health benefit on the host". Probiotics are commonly consumed as part of fermented foods with specially added active live cultures, such as in yoghurt, or as dietary supplements 8 November 2015Clinical Pharm. Biotech. PM7032

3. Probiotics: Lactic Acid Bacteria `Lactic acid bacteria’ includes species of the following genera: Lactobacillus Streptococcus Enterococcus Pediococcus Leuconostoc All these species can produce lactic acid in considerable amounts. They are Gram-positive, cocci or rods, catalase and oxidase negative 8 November 2015Clinical Pharm. Biotech. PM7033

4. Detection of Lactobacillus in Yoghurt by Simple Staining Procedure The MRS formulation was developed by de Man, Rogosa and Sharpe 1 to encourage the growth of the `lactic acid bacteria’ Selectivity of MRS media can be altered by pH adjustment. Lactobacilli will tolerate lower pH levels (pH 5.0-6.5) with pediococci and leuconostocs growing best within this range than streptococci. 8 November 2015Clinical Pharm. Biotech. PM7034

5. Detection of Lactobacillus in Yoghurt by Simple Staining Procedure Lactobacilli are microaerophilic and require incubation at 30⁰C for 5 days or 35⁰C for 3 days. Colonies may be compact or feathery, and are small, opaque and white. Perform simple stain and catalase test on the isolated colonies 8 November 2015Clinical Pharm. Biotech. PM7035

6. Screening for some Protease-containing Drugs

7. Protease activity in alpha-chymotrypsin ampoule, iruxol ointment and digestin syrup Alpha-Chymotrypsin contains a special type of proteases called “endopeptidases”. Chymotrypsin cleaves peptide bonds formed by aromatic residues such as tyrosine, phenylalanine and tryptophan. Iruxol ointment contains a collagenase clostridiopeptidase and other proteases. Digestin syrup has papain, pepsin and sanzyme. 8 November 2015Clinical Pharm. Biotech. PM7037

8. Add alpha chymotrypsin to the first cup Add iruxol ointment to the second cup Add digestin syrup to the third cup Skimmed Milk Agar Plate 8 November 2015Clinical Pharm. Biotech. PM7038

9. The clear zones surrounding the cups are indicative of the proteolytic activity of the preparations 8 November 2015Clinical Pharm. Biotech. PM7039