1. Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister
Development of a Modular Peristaltic Microfluidic Pump and Valve System
BME 272 Group 20:
Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister

2. Microfluidics Minimal reagent consumption Increased speed of reactions
Study of biological phenomena at the single cell level

3. Soft Lithography

4. Current Pumps at VIIBRE
Harvard Pico Plus syringe pumps
$2,000 / pump
Limiting complexity of microfluidic devices

5. Pneumatic Valves Two-layer PDMS device
Flow layer (bottom)
control layer (top)
Thin PDMS membrane deflects into the flow channel when the control channel is pressurized
Low aspect ratios
1:20 for rectangular control channel
1:10 for round flow channel
Unger et al. 2000

6. Pneumatic Valve Design
Flow channel dimension
– 100um wide, 10um tall (round, AZ p4620, mask)
or 5um tall (rectangular, SU8)
Control channel
– valve area (300um by 300um),
control line 100um wide,
25um-30um tall

7. Valve in Action

8. Current Design of Peristaltic Pump
Consists of 4 valves
Round flow channel
Spacing between each valve 100um
Control pressure: 20psi-25psi
Issues
Mechanical stability of membrane
Reusability of pumps
Surface adsorption to PDMS
Flow rates and pressures unknown
Gas diffusion through membrane
Minimize peristalsis

9. Pump in Action

10. Nitrogen or Liquid
Screw or Pneumatic Valves
Christmas Tree Nanophysiometer
Connector
Fluid Flow
Peristaltic Pump

11. Future Work Learn microfabrication procedures from Yuxin
Get Mike and Adam trained in microfabrication (pending IRB approval)
Extension and documentation of LabVIEW pump control system
Build additional valve control box
Characterize valves and pump