1. Biology Mr. Swift Objectives Microscopy Intro to The Optical Microscope The Optical Microscope Questions #1 – 9 Answer 5 questions on slide on blank sheet of paper Day 2: January 22, 2015 Viewing Different Cell Types (Microscopy Lab) Cell Types Closing Review of Main Ideas

2. Why do we use microscopes? Ideas???

4. Compound Light Microscope 1.Eyepieces (Ocular Lenses) Contains magnifying lenses to look through 2.Revolving Nosepiece Holds and turns the objectives into viewing position 3.Objective lenses Low-power objective Contains the lens with low power magnification High-power objective Contains lenses with greater powers of magnification 4.Focus knobs (to move the stage) Coarse adjustment Focuses the image under low power 5.Fine adjustment Sharpens the image under high and low magnification 6.Stage (to hold specimen) Supports the microscope slide 7.Light Source Allows the light to reflect upward through the diaphragm, the specimen and the lenses 8.Diaphragm Regulates the amount of light that passes through the specimen 9.Stage clips Hold the microscope slide in place

5. Magnification The total magnification of the compound light microscope is the product of the power of the eyepieces (oculars) 10 times the objective lens Example: 10 (eyepieces) times 4 (lens) = 40X magnification 10 (eyepieces) times 10 (lens) = 100X magnification 10 (eyepieces) times 40 (lens) = 400X magnification

6. Questions… 1.What part of the microscope is adjusted to change the magnification of the image being viewed? 2.What part of the microscope is used to adjust the amount of light that passes through the specimen? 3.What is the part of the microscope that holds up the objectives and eyepiece called? 4.Calculate the total magnification of the following microscope lenses: 5x eyepiece and 40x objective 10x eyepiece and 100x objective 10x eyepiece and 4x objective 5x eyepiece and 5x objective

7. Objectives Viewing Different Cell Types (Microscopy Lab) Cell Types Closing Review of Main Ideas

8. Microscopy Lab Use your compound light microscope to view 3 prepared slides under low and high power Sketch each image in the circles on your paper (make use of color) Be sure to list: Type of specimen being viewed Total magnification power used Answer the 5 questions on the last lab page

9. Closing and Review

10. Uses of Microscopes Studying cells and tissues Studying and identifying bacteria Examining forensic evidence Determining the health of an ecosystem by identifying organisms And many more uses…

11. Compound Light Microscope 1.Eyepieces (Ocular Lenses) Contains magnifying lenses to look through 2.Revolving Nosepiece Holds and turns the objectives into viewing position 3.Objective lenses Low-power objective Contains the lens with low power magnification High-power objective Contains lenses with greater powers of magnification 4.Focus knobs (to move the stage) Coarse adjustment Focuses the image under low power 5.Fine adjustment Sharpens the image under high and low magnification 6.Stage (to hold specimen) Supports the microscope slide 7.Light Source Allows the light to reflect upward through the diaphragm, the specimen and the lenses 8.Diaphragm Regulates the amount of light that passes through the specimen 9.Stage clips Hold the microscope slide in place