1. Radioimmunoassay, enzymoimmunoassay - principles, practical use.
Laboratory measurement of specific IgE antibodies.
Laboratory measurement of autoantibodies.
Flow cytometry - principles, practical use.
Laboratory methods of assessment of cellular immunity.
Laboratory methods of assessment of humoral immunity.

2. Humoral analytes
Precipitation
Aglutination
Turbidimetry
Nephelometry

6. Imunofixation of serum

7. Humoral analytes
Precipitation
Aglutination
Turbidimetry
Nephelometry

12. ELISA – assessment of specific antibodies

13. RAST

14. ELISA – assessment of antigen

16. Indirect immunofluorescence

17. Asessment of cellular imunity
Number of cells - subpopulations
Phagocytosis
Activation of lymphocytes

19. Flow cytometry

21. Gating of lymphocytes

22. T and Th lymphocytes

23. T and B lymphocytes

24. Activated T lymphocytes

25. http://probes. invitrogen

26. IMPORTANT LEUKOCYTE POPULATIONS
CD3+ T lymphocytes:
mature T lymphocytes %
CD4+ T lymphocytes:
helper T lymphocytes %
CD8+ T lymphocytes:
cytoxic T lymphocytes %
CD25+ T lymphocytes:
activated T lymphocytes %
CD19+ B lymphocytes:
mature B lymphocytes %
CD56+ NK cells:
natural killer cells %
CD14+ cells: monocytes
CD15+ cells: granulocytes
CD38+ cells: plasma cells

27. TESTS OF LYMPHOCYTE FUNCTION
Ability to proliferate
Ability to produce cytokines
TESTS OF PHAGOCYTIC FUNCTION
No of granulocytes
Phagocytar activity of granulocytes
Oxidative burst

28. Organ specific autoantibodies
Anti-thyroidal AB
Anti-pancreatic beta cells AB
Anti-acetylcholine receptor AB
Anti-gonadal AB

29. Systemic autoantibodies
Anti-nuclear AB (ANA, ENA)
Rheumatoid factor
Anti-neutrophil cytoplasma AB (ANCA)
Anti-phospholipid AB (APA)

30. Non-organ-specific autoantibodies in organ-localized autoimmune diseases
ANCA (non-specific inflammatory bowel disease) //+ASCA//
ATG (coeliac disease)
SS-A, SS-B (Sjögren syndrome)