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Published byHilary Davis Modified over 9 years ago
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Radioimmunoassay, enzymoimmunoassay - principles, practical use.
Laboratory measurement of specific IgE antibodies. Laboratory measurement of autoantibodies. Flow cytometry - principles, practical use. Laboratory methods of assessment of cellular immunity. Laboratory methods of assessment of humoral immunity.
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Humoral analytes Precipitation Aglutination Turbidimetry Nephelometry
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Imunofixation of serum
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Humoral analytes Precipitation Aglutination Turbidimetry Nephelometry
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ELISA – assessment of specific antibodies
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RAST
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ELISA – assessment of antigen
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Indirect immunofluorescence
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Asessment of cellular imunity
Number of cells - subpopulations Phagocytosis Activation of lymphocytes
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Flow cytometry
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Gating of lymphocytes
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T and Th lymphocytes
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T and B lymphocytes
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Activated T lymphocytes
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http://probes. invitrogen
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IMPORTANT LEUKOCYTE POPULATIONS
CD3+ T lymphocytes: mature T lymphocytes % CD4+ T lymphocytes: helper T lymphocytes % CD8+ T lymphocytes: cytoxic T lymphocytes % CD25+ T lymphocytes: activated T lymphocytes % CD19+ B lymphocytes: mature B lymphocytes % CD56+ NK cells: natural killer cells % CD14+ cells: monocytes CD15+ cells: granulocytes CD38+ cells: plasma cells
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TESTS OF LYMPHOCYTE FUNCTION
Ability to proliferate Ability to produce cytokines TESTS OF PHAGOCYTIC FUNCTION No of granulocytes Phagocytar activity of granulocytes Oxidative burst
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Organ specific autoantibodies
Anti-thyroidal AB Anti-pancreatic beta cells AB Anti-acetylcholine receptor AB Anti-gonadal AB
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Systemic autoantibodies
Anti-nuclear AB (ANA, ENA) Rheumatoid factor Anti-neutrophil cytoplasma AB (ANCA) Anti-phospholipid AB (APA)
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Non-organ-specific autoantibodies in organ-localized autoimmune diseases
ANCA (non-specific inflammatory bowel disease) //+ASCA// ATG (coeliac disease) SS-A, SS-B (Sjögren syndrome)
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