1. DNA Replication IB Topic 3.4 Page 65

2. Answers to your homework (#8-11 page 65)  8. The phosphate groups and deoxyribose sugars within DNA molecules are constant. There is no message written within their presence. The only message is in the order of the nucleotides as given by the nitrogenous bases.

3. Answers Continued  9. Compare your drawing to figure 3.12  10. TAGGCTAGAGTCCTT  11. A. 30% cytosine B. 20% adenine

4. Replication – How DNA copies itself  In order that a copy of each chromosome can pass into the daughter cells at cell division, the DNA of the chromosomes must first be copied (replicated) Why is this important? When does this happen?  DNA carries the genetic message Replication must be extremely accurate Why?

5. DNA Replication is Semi-Conservative  At the end of DNA replication, we have two new strands of DNA Double helix shape  Both (new) DNA molecules are composed of an original strand and a newly synthesized strand Semi-conservative  Because half of a pre-existing DNA molecule is always conserved (saved)

6. Steps of Replication  The DNA double helix unwinds  The hydrogen bonds holding the strands together are broken  The enzyme helicase (and some others) is involved in these steps and holds the strands apart while replication occurs

7. Steps of Replication  Both strands act as templates  New nucleotides (complementary base pairing!) line up opposite the bases of the exposed strand A = T & C = G  The sequence on one DNA strand determines the sequence of the other strand They’re complementary

8. Steps of Replication  Hydrogen bonds hold together the complementary bases  The sugar/phosphate groups of adjacent nucleotides of the new strand condense together Reaction is catalyzed by the enzyme DNA polymerase

9. DNA Replication Tutorial  http://www.wiley.com/college/pratt /0471393878/student/animations/d na_replication/index.html http://www.wiley.com/college/pratt /0471393878/student/animations/d na_replication/index.html

10. Additional DNA Replication Animations  http://www.stolaf.edu/people/giann ini/flashanimat/molgenetics/dna- rna2.swf http://www.stolaf.edu/people/giann ini/flashanimat/molgenetics/dna- rna2.swf  http://highered.mcgraw- hill.com/sites/0072437316/student_ view0/chapter14/animations.html# http://highered.mcgraw- hill.com/sites/0072437316/student_ view0/chapter14/animations.html#