1. StainsStains

2. Cheek Cells Without Stain

3. Cheek Cells with Simple Stain (Methylene Blue)

4. Simple Stain: Negative Stain

5. Differential Stains: Gram Stain Color of Gram + Color of Gram – Primary stain: Crystal violet Purple Mordant: Iodine Purple Decolorizing agent: Alcohol-acetone PurpleColorless Counterstain: Safranin PurplePink

6. Gram Positive and Gram Negative Bacteria

7. Prokaryotic Cell Drawing

8. Outer membrane Peptidoglycan GRAM NEGATIVE GRAM POSITIVE

9. Capsule Stain

11. Figure 3.12a-c

12. Acid-Fast and Non Acid-Fast

13. Endospore Stain: Ziehl-Neelsen

14. Figure 3.12a-c

15. Endospore Stain

16. Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST SPORE STAIN PRIMARY STAINCrystal violet Carbol Fuschia Malachite green MORDANTIodine Heat DECOLORIZERETOH or acetone: Cell is purple ETOH or acetone: Cell is clear Acid alcohol Water COUNTER-STAINSafranin: Cell is purple Safranin: Cell is pink Methylene Blue Safranin: Spores are green Cell is pink

17. Flagella Stain

19. Figure 3.12a-c

20. Monotrichous Lophotrichous Amphitrichous Peritrichous Names for flagella arrangement