1. Review for Exam 4 Jones and Bartlett Publishers © 2005

2. Terms to know: Ch. 10 aminoacyl-tRNA synthetasepolycistronic DNA anticodonpolysome E/P/A sites of ribosomeprimary transcript charged tRNApromoter coding sequenceRNA processing exon/intronsnRNP exon-shuffle modeltranslation lariat structure wobble polyA tail

3. Terms to know: Ch. 11 anti-sense RNAenhancer aporepressor epigenetic attenuationhousekeeping genes beta-galactosidaseinducer cAMP-CRP complexoperator chromatin-remodeling complexoperon constitutive mutantrepressor CpG islandsilencer DNA methylationantibody parts

4. Terms to know: Ch. 12 BACgenomics bioinformatics library Blunt/sticky end proteomics cloned DNA reporter gene cDNASTS contigTi plasmid DNA microarraytransgenic organism ESTvector knockout mutationpolylinker

5. Discussion topics Know the details of transcription and translation – enzymes involved, what is produced, where the process happens, etc. (about 50% of the exam). Know why the genetic ‘code’ is said to be degenerate (or redundant), and what wobble is. Know the different ways genetic expression is regulated. Know the lac operon details. In addition to the terms in Chapter 12, know how reverse transcriptase is used to make cDNA, and how this is helpful. Also, know the ten fill-in-the-blank questions for each of the three chapters.