2. © Mark E. Damon - All Rights Reserved

3. Another Presentation © 2002 - All rights Reserved nygiantsbigblue@yahoo.com

4. © Mark E. Damon - All Rights Reserved Directions: Scroll through the presentation and enter the answers (which are really the questions) and the questions (which are really the answers). Enter in the categories on the main game boards. As you play the game, click on the TEXT DOLLAR AMOUNT that the contestant calls, not the surrounding box. When they have given a question, click again anywhere on the screen to see the correct question. Keep track of which questions have already been picked by printing out the game board screen and checking off as you go. Click on the “Game” box to return to the main scoreboard. Enter the score into the black box on each players podium. Continue until all clues are given. When finished, DO NOT save the game. This will overwrite the program with the scores and data you enter. You MAY save it as a different name, but keep this file untouched!

5. © Mark E. Damon - All Rights Reserved Round 1Round 2 Final Jeopardy TEAM 1 TEAM 2 TEAM 3

6. © Mark E. Damon - All Rights Reserved PCR Gels and other stuff… Recombi nant DNA Restricti on Maps Stem Cells Cloning & Misc Trivia $100 $200 $300 $400 $500 Final Jeopardy Scores

7. © Mark E. Damon - All Rights Reserved $100 This is abbreviated PCR

8. © Mark E. Damon - All Rights Reserved $100 What is Polymerase Chain Reaction Scores

9. © Mark E. Damon - All Rights Reserved $200 Define Polymerase Chain Reaction (what does it do?)

10. © Mark E. Damon - All Rights Reserved $200 Uses enzymes and heat to amplify small amounts of DNA Scores

11. © Mark E. Damon - All Rights Reserved $300 Give three ingredients for PCR and their purpose

12. © Mark E. Damon - All Rights Reserved $300 DNA Primers Taq Polymerase dNTPs Buffer Thermal Cycler DNA Primers Taq Polymerase dNTPs Buffer Thermal Cycler Scores

13. © Mark E. Damon - All Rights Reserved $400 What are primers (include what end they are complementary to)?

14. © Mark E. Damon - All Rights Reserved $400 Short pieces of DNA that are complementary to the 3’ ends of our target DNA Scores

15. © Mark E. Damon - All Rights Reserved $500 List the three stages of a PCR cycle. Include: 1.Approx. Temp 2. Name of the Stage 3.What happens during the stage List the three stages of a PCR cycle. Include: 1.Approx. Temp 2. Name of the Stage 3.What happens during the stage

16. © Mark E. Damon - All Rights Reserved $500 Denature (95C) – strands come apart Anneal (50C) – primers attach Extension (70C) – taq pol. Extends DNA Denature (95C) – strands come apart Anneal (50C) – primers attach Extension (70C) – taq pol. Extends DNA Scores

17. © Mark E. Damon - All Rights Reserved $100 What charge is DNA?

18. © Mark E. Damon - All Rights Reserved $100 Negative Scores

19. © Mark E. Damon - All Rights Reserved $200 Finish this sentence: The ________ the DNA, the ________ it will travel

20. © Mark E. Damon - All Rights Reserved $200 Larger ; Slower Scores

21. © Mark E. Damon - All Rights Reserved $300 List three ingredients of electrophoresis and describe their purpose

22. © Mark E. Damon - All Rights Reserved $300 Agarose Gel Electric Current Buffer DNA Dye Chamber Agarose Gel Electric Current Buffer DNA Dye Chamber Scores

23. © Mark E. Damon - All Rights Reserved

24. $400 In gel electrophoresis, we often use ethidium bromide as our dye. How does ethidium bromide work?

25. © Mark E. Damon - All Rights Reserved $400 It is a intercalating agent – it inserts itself in the DNA and it shines under a UV light – we can see where the DNA is Scores

26. © Mark E. Damon - All Rights Reserved $500 In DNA sequencing, we use dideoxynucleotides to stop the replication of the DNA. What is it about these molecules that stops replication (hint: I mentioned it very briefly)

27. © Mark E. Damon - All Rights Reserved $500 They are missing a 3’ -OH group Scores

28. © Mark E. Damon - All Rights Reserved $100 This is the term for DNA from different sources that has been combined into one new piece

29. © Mark E. Damon - All Rights Reserved $100 Recombinant DNA Scores

30. © Mark E. Damon - All Rights Reserved $200 What are restriction enzymes?

31. © Mark E. Damon - All Rights Reserved $200 Enzymes that cut DNA at specific sequences Scores

32. © Mark E. Damon - All Rights Reserved $300 If I treat two pieces of DNA with EcoRI, will I be able to recombine them? Why?

33. © Mark E. Damon - All Rights Reserved $300 Yes ; DNA treated with the same restriction enzymes produces complementary sticky ends Scores

34. © Mark E. Damon - All Rights Reserved $400 Explain the process of using recombinant DNA technology to manufacture insulin

35. © Mark E. Damon - All Rights Reserved $400 Scores

36. © Mark E. Damon - All Rights Reserved $500 What are the three components of a plasmid. List and describe

37. © Mark E. Damon - All Rights Reserved $500 1.Replicator 2.Selectable Marker 3.Cloning Site 1.Replicator 2.Selectable Marker 3.Cloning Site Scores

38. © Mark E. Damon - All Rights Reserved $100 What would result if we cut with EcoR1?

39. © Mark E. Damon - All Rights Reserved $100 One, 15.4 kb fragment Scores

40. © Mark E. Damon - All Rights Reserved $200 What would result if you cut with HindIII?

41. © Mark E. Damon - All Rights Reserved $200 Two fragments: 7.5 & 7.9 kb Scores

42. © Mark E. Damon - All Rights Reserved $300 What would result if you cut with ClaI?

43. © Mark E. Damon - All Rights Reserved $300 2 Fragments; 3.6 & 11.8 kb Scores

44. © Mark E. Damon - All Rights Reserved $400 What would result if you cut with Bg/II?

45. © Mark E. Damon - All Rights Reserved $400 3 fragments:.25, 4.6, & 10.55 kb Scores

46. © Mark E. Damon - All Rights Reserved $500 What would result if you treated with EcoRV?

47. © Mark E. Damon - All Rights Reserved $500 6 Fragments:.65,.54, 3.1,.81, 2.4, 7.9 Scores

48. © Mark E. Damon - All Rights Reserved $100 What is differentiation?

49. © Mark E. Damon - All Rights Reserved $100 The process of a cell becoming specialized Scores

50. © Mark E. Damon - All Rights Reserved $200 List two differences between adult and embryonic stem cells

51. © Mark E. Damon - All Rights Reserved $200 Embryonic: from embryos ; can become any cell type ; made through cloning ; most potential Adult: from adults, infants, kids ; multipotent ; limited cells types ; found in limited locations Embryonic: from embryos ; can become any cell type ; made through cloning ; most potential Adult: from adults, infants, kids ; multipotent ; limited cells types ; found in limited locations Scores

52. © Mark E. Damon - All Rights Reserved $300 List and describe the three levels of cell potency

53. © Mark E. Damon - All Rights Reserved $300 Totipotent ; Pluripotent ; Multipotent Scores

54. © Mark E. Damon - All Rights Reserved $400 What are iPS cells?

55. © Mark E. Damon - All Rights Reserved $400 Induced Pluripotent Stem Cells: re-engineered stem cells that act like ESC - made through inserting either 4 genes or 4 gene products Scores

56. © Mark E. Damon - All Rights Reserved $500 What is asymmetric division?

57. © Mark E. Damon - All Rights Reserved $500 Stem Cell Self-Renewal – stem cells divide and one goes on to differentiate. The other remains a stem cell Scores

58. © Mark E. Damon - All Rights Reserved $100 What is cloning?

59. © Mark E. Damon - All Rights Reserved $100 The process of creating a genetically identical organism through nonsexual means Scores

60. © Mark E. Damon - All Rights Reserved $200 What is epigenetics?

61. © Mark E. Damon - All Rights Reserved $200 Gene expression caused by mechanisms other than DNA sequence Scores

62. © Mark E. Damon - All Rights Reserved $300 According to Wikipedia, what percentage of cab drivers in NYC are foreign-born

63. © Mark E. Damon - All Rights Reserved $300 82 Percent Scores

64. © Mark E. Damon - All Rights Reserved $400 How long does it take the average person to fall asleep?

65. © Mark E. Damon - All Rights Reserved $400 7 minutes Scores

66. © Mark E. Damon - All Rights Reserved $500 What is acetylation and methylation?

67. © Mark E. Damon - All Rights Reserved $500 Mechanisms of Epigentics: Acetylation = turns on the gene Methylation = turns off the gene Mechanisms of Epigentics: Acetylation = turns on the gene Methylation = turns off the gene Scores

68. © Mark E. Damon - All Rights Reserved Scores Cloning Final Jeopary Question

69. © Mark E. Damon - All Rights Reserved As you are walking down the street, you hear someone mention that “a clone is an exact copy of another organism in every way including DNA, appearance, and personality.” You know this is not correct! Give four reasons for why clones are not exact replicas.

70. © Mark E. Damon - All Rights Reserved Epigenetics (methylation & acetylation) mtDNA X-inactivation Nature vs. Nurture (different environment / experiences) Epigenetics (methylation & acetylation) mtDNA X-inactivation Nature vs. Nurture (different environment / experiences) Scores