1. Energy Transfer/Biomass AP BIOLOGY LAB Estimating Net Primary Productivity (NPP) in Plants

2. Step 1 Energy Flow Diagram Energy Flow Into and Out of a Plant Energy/Biomass In Plant (on day __) kcal from light energy kcal lost as waste kcal for respiration biomass

3. Steps 2- 3 Design lab to determine change in biomass over time = productivity Grow plants – min. of 20 At 7 days growth take wet mass and dry mass Use proper procedure for drying plants At 14 days growth take wet mass and dry mass readings Before collecting data: Predict how biomass/energy will change over the course of the plant’s life cycle. – Graphically show your prediction

4. Step 4 Calculating NPP Determine the average (mean) grams of biomass added PER PLANT over the period of growth Convert grams/day to NPP which is kcal/day Question: Explain why this is Net Primary Productivity and not Gross productivity?

5. Step 5 Analysis of data Discuss why the mass of the dry plants is the measurement used for primary productivity instead of mass of living plant. Determine the percentage of the living plants biomass.

6. Step 6 Data and Analysis Age of plantWet mass of 10 plants Dry mass of 10 plants Percent biomass Energy (g biomass X 4.35kcal) for 10 plants NPP Per day Per plant

7. Step 6 Data and Analysis (continued) Reconstruct energy flow diagram using real data. Support your new energy flow diagram with an explanation and evidence to support your numbers. Be sure to address uncertainties and limitations of your data.

8. Energy Transfer/Biomass AP BIOLOGY LAB Estimating Energy Flow Between Producers and Primary Consumers

9. Step 1