1. Tissues & Junctions Lecture 34 Chapter 21 - Part 1

2. A) TISSUES

3. What is tissue? Biological tissue is a collection of similar cells and the intercellular substances surrounding them. There are four basic kinds of tissue in the body: 1.epithelium 2.connective tissues –including adipose tissue, blood, bone, and cartilage 3.muscle tissue 4.nerve tissue

4. 21_02_tissues.jpg With most multicellular organisms there are different types of cells, which are organized in to discrete groupings called tissues

5. Plants

6. 21_05_model_plantwall.jpg Plant cells produce a primary cell wall followed by a secondary cell wall. The cell wall - which is on the outside of the plasma membrane - is made of cellulose - a polysaccharide

7. 21_06_cellulose.jpg The cell wall will only allow the cell to grow in one direction, which is dependant upon the orientation of the fibers that were laid down during the production of the primary cell wall. A) Here the plant tissue may only expand in a vertical direction B) Here the plant tissue may only expand in a horizontal direction

8. 21_07_Microtubules.jpg The celulose synthase complex, that is embedded in the plasma membrane, oozes out cellulose microfibrils

9. Animals

10. 21_08_Extracellu_matrix.jpg Animal cells do not use cellulose. They instead have a protein extracellular matrix of collagen, which has great tensile strength. Animals have just 4 classes of tissue - connective, epithelial, nervous, and muscular (the last three are basically similar).

11. 21_09_Collagen_fibrils.jpg Collagen is made of many bundles of a simple polypeptide

12. 21_11_elastic_skin.jpg Mutations in the arrangement of these fibers may result in various abnormal outcomes.

13. 21_13_align_collagen.jpg Fibroblasts are the cells that secrete collagen and also the ones that maintain it.

14. 21_14_Integrins_link.jpg The interlinks between cells is made between collagen fibers and the actin filaments of cells using transmembrane and adaptor proteins known as integrins How are cells held together? Fibronectin - is a linker protein

15. 21_17_epithelial_sheet.jpg Epithelial sheets and cell-cell junctions

16. 21_18_sheet_polarized.jpg Most tissue cells have a polarized structure. They rest on the basal lamina. The basal lamina is just a layer of type IV collagen, plus other molecules. Lung Tissue

17. 21_20_polarize_line_gut.jpg The intestine is made of two main cell types. The absorptive cells take in nutrients and the goblet cells secrete mucus.

18. 21_21_cell_cell_junction.jpg Animal cells are held together by a number of types of cell- cell junctions. a) Tight Junctions - b) Adherens junction - c) desmosomes d) gap juctions - e) hemidesmosomes

19. 21_22_Tight_junctions.jpg Tight junctions prevent the passage of solutes between cells. They also allow the cell to maintain discrete types of plasma membrane regions

20. Tight junctions consist of proteins, for example, claudins, occludins and junctional adhesion molecules that are anchored in the membranes of two adjacent cells and interact with each other to hold the cells together and prevent other molecules from passing between them.

22. 21_23_Cadherin.jpg Adheren junctions and desmosome junctions are build on a common theme - they make use of transmembrane proteins that belong to the cadherin family - these molecules from adjacent cells link directly to each other. actin or intermediate filament

23. 21_24_Adherens_junct.jpg Adheren junctions form large deposits as belts permitting adjacent cells to transmit mechanical forces

24. 21_25_tube_or_vesicle.jpg Vital part in development

25. 21_26_Desmosomes.jpg Desmosomes

26. 21_27_Hemidesmosome.jpg Hemidesmosomes

27. Gap junctions permit the free passage of small ions and small water soluble molecules to pass. However, they can be closed on demand. Plants have these too.

28. 21_30_plasmodesmata.jpg

29. A little perspective… The Inner Life of a Cell - Short Version Annotated Version