1. CONTACT NUMBERS TEL:(202)745-8330 FAX: (202)462-2006 EMAIL: RAJ.LAKSHMAN@MED.VA.GOV DR. RAJ LAKSHMAN PROFESSOR OF BIOCHEMISTRY, MOLECULAR BIOLOGY & MEDICINE

2. Mechanisms of Actions of Alcohol & Omega3-Fatty Acids.  To delineate the mechanisms of actions of alcohol and omega3-fatty acids in altering the post-translational modifications of glycoproteins and their functions. Light & Heavy Alcohol, Paraoxonase & Oxidized LDL Status.  The major goal of this project is to evaluate the benefits and deleterious effects of moderate and heavy drinking on cardiovascular risk factors DR. LAKSHMAN’S FUNDING NIH GRANTS

3. ST6 Gal 1 mRNA Protein Complex UNSTABLE ST6 Gal 1 mRNA Normal CH Efflux LCAT Apo-E Cholesterol Loaded Macrophage Asialo- conjugates PL Free CH HDL3 HDL2 LIVER CELL BLOOD COMPARTMENT ST6 Gal 1 3’UTR Binding Protein (~41kD) Normal ST6Gal1 Sialo- conjugates X CHE VLDL Associates weakly with Associates strongly with Cholesterol uptake ω3-Fatty Acids Actions of Alcohol & Omega3-Fatty Acids

4. HDL-PON LDL OX-LDL Plaque Macrophage Inactive products Up-regulated by light ethanol Down-regulated by heavy ethanol Foam Cell Bimodal Action of Alcohol on PON & CAD Status