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KS + Ima + Zvad % of positive cells (ANV+) Cells positive for SA-β-gal (%) Cells positive for SA-β-gal (%) % of positive cells (ANV+) Supplementary Figure.

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Presentation on theme: "KS + Ima + Zvad % of positive cells (ANV+) Cells positive for SA-β-gal (%) Cells positive for SA-β-gal (%) % of positive cells (ANV+) Supplementary Figure."— Presentation transcript:

1 KS + Ima + Zvad % of positive cells (ANV+) Cells positive for SA-β-gal (%) Cells positive for SA-β-gal (%) % of positive cells (ANV+) Supplementary Figure 1 n = 8 A-B- C-D- KS + Ima + Zvad

2 K562 K562 + Ima K562 + Ima + Zvad Supplementary Figure 1E BD Drullion et al AC

3 Hsp60 LC3B KSKS shATG7 Ima BafA1 - + - + - + - + -- ++ -- ++ Hsp60 LC3B Hsp60 LC3B KS KS shATG7 HBSS - BafA1 06h 12h9h 12h 06h 12h9h 12h HBSS + BafA1 Supplementary Figure 2 A- B-

4 KSKS shATG7 Control Ima Supplementary Figure 2C

5 K562 sh ATG7 K562 sh ATG7 + Ima K562 sh ATG7 + Ima + Zvad A B C D Supplementary Figure 3A Drullion et al

6 Ima----++++ Tetra--++ sh casp3sh casp9 Casp3 Casp9 p21 p27 hsp60 Cleaved Casp3 % of positive cells (ANV+) Supplementary Figure 4 n = 3 A- B-

7 Cells positive for SA-  -Gal (%) Supplementary Figure 4 n = 3 C-

8 sh-scr sh-Bcn control sh-ATG5 1245 ATG5 Hsp 60 Beclin 1 Hsp 60 Supplementary Figure 5 A- Ima Zvad -++ - -+ Mean fluorescence intensity n = 3 *p<0.05 * *

9 % of positive cells (ANV+) n = 4 *p<0.05 * Ima Zvad -++ - -+ B- Cells positive for SA-b-Gal (%) n = 4 *p<0.05 *** Ima Zvad -++ - -+ C- * Supplementary Figure 5

10 KS KS sh-scr Control Ima +ZVad KS sh-Bcn KS sh-ATG5 Supplementary Figure 5 D-


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