1. Supplemental Figure I: Representative blots to support Figure 5A WistarGK AMPK β-actin LGHG 1hHG 4h AMPK β-actin HG 1h LG HG 4h pAMPK β-actin LGHG 1h HG 4h pAMPK β-actin LG HG 1h HG 4h peNOS β-actin LGHG 1hHG 4h LG peNOS β-actin HG 1h HG 4h PGC-1α LG HG 1h HG 4h β-actin PGC-1α LG HG 1h HG 4h VDAC1 β-actin LG HG 1hHG 4h VDAC1 β-actin HG 1h LG HG 4h

2. WistarGK Complex II Complex V Complex III Complex IV Complex I β-actin LG HG 1h HG 4h Complex V Complex III Complex IV Complex II Complex I LGHG 1hHG 4h β-actin Supplemental Figure II: Representative blots to support Figure 5A

3. MnSOD LG HG 1h HG 4h β-actin UCP3 HG 4hHG 1hLG MnSOD β-actin LG HG 1h HG 4h UCP3 LGHG 1h HG 4h β-actin Wistar GK Supplemental Figure III: Representative blots to support Figure 5B

4. Mfn1 β-actin LG HG 1h HG 4h LG HG 1h HG 4h β-actin Mfn2 OPA1 β-actin LG HG 1h HG 4h Mfn1 β-actin LG HG 1h HG 4h Mfn2 β-actin LG HG 1h HG 4h OPA1 β-actin LG HG 1h HG 4h WistarGK Supplemental Figure IV: Representative blots to support Figure 5D

5. GKWistar LG HG 1h HG 4h Beclin β-actin LG p62 β-actin HG 1hHG 4h LC3-I β-actin LC3-II LGHG 1hHG 4h β-actin Atg7 LGHG 1hHG 4h Beclin β-actin LGHG 1hHG 4h β-actin p62 LGHG 1hHG 4h LC3-II LGHG 1hHG 4h LC3-I β-actin Atg7 LGHG 1hHG 4h β-actin Supplemental Figure V: Representative blots to support Figure 5D